Dissertation > Excellent graduate degree dissertation topics show

Synthesis and Application of Molecularly Imprinted Polymers for Selective Capturing Antibiotics Containing Beta-diketone Structure

Author: ZuoShanShan
Tutor: TongShangLun
School: Zhejiang University
Course: Environmental Science
Keywords: molecularly imprinted polymer solid-phase extraction (SPE) β-diketone antibiotics high performance liquid chromatography (HPLC) UV determination
CLC: X132
Type: Master's thesis
Year: 2011
Downloads: 168
Quote: 0
Read: Download Dissertation

Abstract


Nowadays, a large amount of antibiotics especially that with the P-diketone struature are used in the treatment and prevention of illness in human, veterinary and aquaculture, and as growth promoter in veterinary and aquaculture. Nevertheless, a great part of antibiotics and their active metabolite are excreted into the environment. The existence of antibiotics and their active metabolite in environment will probably influence the micro organization of the natural environment and lead to resistance in bacteria, and cross-resistance between human and veterinary. Hence research on the residue of antibiotics in environment and their environmental risk and to establish a sensitive and rapid pharmaceutical determination method is of great importance in environmental treatment and ecological protection.In the first part, the applications of some separation and analysis methods of antibiotics in environmental samples for the detection emerged in recent years were summarized in detail. The synthesis and application of molecularly imprinted polymer were also reviewed.In the second part, we chose ITA as functional monomer for it could strongly coordinate with metal ions. We successfully prepared the MIP using the ITA-Fe3+-CIP complex which can absorb all antibiotics that withβ-diketone structure.In this research, we took cyclic voltammetry, dynamic absorption, equilibrium absorption to identify the MIP could absorb both of the template and other molecules withβ-diketone structure and to confirm the best prepare condition. Varies preparation conditions were investigated for they are the key factor for adsorption capacity. The MIP was prepared when the ITA-Fe3+-CIP ratio and the proportion of EDMA were 1:1:4 and 70%, respectively. Its AC and TE factor were 8.37 and 7.68, respectively. Equilibrium dissociation constant at higher affinity site and lower affinity site were KD1=120.63μmol L-1 and KD2=3.31μmol L-1, respectively. Maximum apparent adsorption capacity at higher affinity site and lower affinity site were Qmax1= 141.13μmol g-1 and Qmax2=19.97μmol g-1.In the third part, a high-performance liquid chromatography (HPLC)-ultraviolet detection method, combined with MIP-solid-phase extraction (MIPSPE), was developed for the determination of nineβ-diketone antibiotics including PIP, FLR, NOR, OFL, TC, OTC, CIP, ENR, LOM in surface and waste water samples. Extraction of the nine components was carried out with the MIP cartridge. The water sample was added in Na2EDTA and adjusted to pH=6.5. Then the cartridge was eluted by a mixture of methanol and acetic acid (3mL,6:1/v:v). Separation of the nine components was achieved by using an Agilent HPLC and detected by a UV detector at a wavelength of 275 nm. Recoveries of the nine components in the spiked sewage sample were between 75.2 and 97.9%with a relative standard deviation of less than 7.1%. The limits of detection (S/N=3) were between 1.2×10-8～1.0×10-7 mol L-1. The proposed method was applied to determine the waste water of Sibao sewage treatment plant (STP). Results indicated that four components were detected in influent of Sibao STP, the concentrations were between 3.4～67.8×10-10 mol L-1. While in effluent of Sibao STP there were three components were detected, the concentrations were between 5.0～47.2×10-10 mol L-1.

Related Dissertations

  1. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  2. The Influence of Antibiotics on Yogurt During the Fermentation,TS252.54
  3. Green Econazole on the Production Performance and Meat Quality of Finishing Pigs,S828.5
  4. Study on Synthetic of BINOL, Amino Acid and Derivatives Molecularly Imprinted Polymers and Their Application in the Chiral Resolution,O631.3
  5. Strain Screening of Hainanmycin Production and Fermentation Process Optimization,TQ927
  6. Molecularly Imprinted Polymer Microspheres and Evaluation,O631.3
  7. For drug ingredients isolated molecularly imprinted polymer microspheres preparation , properties and application of testing,O631.3
  8. Isolation and Purification of a Novel Antifungal Antibiotic and Optimization of Its Fermentation Conditions,TQ927
  9. Cyclothiazomycin Variants Generated by Site Directed Mutagenesis of Prepeptide,Q93
  10. Detection and Change of CaV1.3 Calcium Channel and Three Apoptotic Factors in Acute Drug-induced Lesion of Inner Ear,R764
  11. New ferrocene derivatives Synthesis, Characterization and Electrochemical Properties,O627.81
  12. Clinical Analysis of Mycoplasma Pneumoniae and Its Role in Immunological Pathology,R563.1
  13. The Competitive Inhibition Assay and Experimental Study on Antibiotics and Melamine Residues in Milk,TS252.7
  14. Preparation of Quinolones Molecularly Imprinted Polymer Microspheres and Study of Its Specific Adsorption Performance,O631.3
  15. The pyrethroid pesticide immune and chromatographic analysis method,TQ450.2
  16. Research on the Synthesis and Properties of EGCG-molecularly Imprinted Polymer,O631.3
  17. Preparation of Molecularly Imprinted Polymers Via Atom Transfer Radical Polymerization,O631.3
  18. Synthesis and Molecular Recognition of Flavonoids Functional Factors of Traditional Chinese Medicines-Imprinted Polymer,O631.3
  19. A Comparison of the Efficacy of Continuous Administration Using Minipump and Fractional Administration of Antibiotic in Treating Lower Respiratory Tract Bacterial Infections,R96
  20. The Study of Capillary Biomembrane Chromatography in Interactions between β-lactam Antibiotics and Biomembrane,R96
  21. Clinical Research on External Fixator in the Treatment of Infection after Open Fractures of Femur,R687.3

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Chemistry > Environmental Analytical Chemistry
© 2012 www.DissertationTopic.Net  Mobile