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In this paper,the anther study was carried on and the thermo-sensitive genie male sterile lines and hybrid combinations (Di D4, Da 107, Di D4×Hong Qunlun, Da107×Niu Fanqie) were used as the experiment materials, in which included three aspects:(1) The test on anther callus induction of thermo-sensitive genic male sterile lines of Tomato; (2) The test on anther callus differentiation of thermo-sensitive genic male sterile lines of Tomato; (3) The cytology observa -tion on anther and callus of thermo-sensitive genic male sterile lines of Tomato. The main resea -rch results indicated as following:1 The test results on anther callus induction of thermo-sensitive genic male sterile lines of tomato:(1) In the comparison test of callus indiction of hormone cominations with double factors of 2,4-D combination with 6-BA or KT, the effect of conbination 2,4-D1.0mg/L+6-BA 1.0 mg/L was the best. The anther callus induction of thermo-sensitive genie male sterile lines of tomato reached to the highest. It was Da 107 (35.80%), Da107×Niu Fanqie (25.85%), Di D4 (36.65%), Di D4×Hong Qunlun (28.13%) respectively.(2) In the basic of 2,4-D1.0mg/L+6-BA1.0mg/L, the comparison test of hormone combinations with three factors was carried, in which combinated with CPPU, TDZ, KT or ZT, the effect of combination 2,4-D1.0mg/L+6-BA1.0 mg/L+KT1.5mg/L was the best, the callus induction rate was Da107 (65.07%), Da107×Niu Fanqie (43.10%), Di D4 (66.20%), Di D4×Hong Qunlun (46.76%) respectively.(3) The test results of pretreatment of different time in 4℃showed that optimal pretreatment time of the parent was for 72h, the callus induction rate was Da 107 (70.70%), Di D4(71.55%), and the time of callus induction was the earliest, the pretreatment time of the hybrid groups was for 96h, the callus induction rate was Da107×Niu Fanqie (51.14%), Di D4xHong Qunlun (53.71%) respectively, and callus induction rate reached peek.(4) In the comparative test of three basic mediums, the MS medium callus induction effect of thermo-sensitive genic male sterile lines of tomato better than N6, white. induction rate reached respectively Da107(64.79%), Da107×NiuFanqie(43.66%), Di D4(65.63%), Di D4xHong Qunlun(47.32%).(5) In the light processing of different time, especially effect of anther callus induction of all the dark processing was the highest, and the browning rate was the lowest, callus was a soft and glossy, vigorous growth, the callus induction rate was Da107(64.79%), Da107×NiuFanqie(43.50%), DiD4(65.54%), DiD4xHongQunlun (47.46%) respectively.(6) Undering the same hormone combination conditions, MS+2,4-D1.0 mg/L+6-BA 1.0 mg/L+KT1.5 mg/L combination with sugar concentration was for 35g/L, in which was the best induction effect, the callus induction rate was Dal 07 (64.44%), Da107×Niu Fan qie (43.61%), DiD4(66.94%), DiD4xHongQunlun (47.50%) respectively.(7) In prevent browning trials of tomato anther callus, effect of prevent browing was the best, in which PVP concentration was 1.0g/L. the anther callus browning rate:Da107 (23.01%), Da107×Niu,Fanqie(39.20%), DiD4(20.74%), DiD4xHongQunlun (37.22%) respectively, callus induction rate:Da107 (73.58%), Da107×Niu Fanqie (52.29%), Di D4(75.28%), Di D4×Hong Qunlun(56.57%) respectively.(8) In the comparative test of amino acids, optimal induction effect of the parent anther callus was for P2 processing (2,4-D1.0 mg/L+6-BA1.0 mg/L+KT1.5 mg/L+Pro500mg/L+ GLy50mg/L), callus induction rate:Da 107(81.49%), DiD4(83.43%) respectively; optimal induction effect of the hybrid groups anther callus was for P3 processing (2,4-D1.0 mg/L+ 6-BA1.0mg/L+KT1.5mg/L+Pro500mg/L+Gly75mg/L), callus induction rate:Da107×Niu Fanqie (59.23%)%, Di D4×Hong Qunlun(64.74%) respectively.2 Test result on anther callus differentiation of thermo-sensitive genic male sterile lines of tomato.(1) In the comparative test of callus differentiation of different hormones combina -tions with NAA,6-BA, KT, CPPU, ZT and TDZ, the result showed:the best hormone combinaton for bud differention was S5(MS+NAA0.1mg/L+KT1.0mg/L+6-BA2.0mg/L), V3 (MS+NAA 0.1mg/L+6-BA2.0mg/L+TDZ0.04mg/L),W4-W5(MS+NAA0.1mg/L+6-BA2.0mg/L+ZT(0.4-0.6))mg/L, differtntiation rate respectively:S5(Da107 2.22%, Di D4 4.44%); V3(Da107 4.44%, Da107×NiuFangqie2.22%, Di D4 2.22%); W4-W5(Da1076.67%, Di D4 4.44%, Da107×NiuFangqie2.22%, Di D4xHong Qunlun2.22%).(2) In differentiation comparatives test of different concentrations of coconut juice, MS+NAA0.1 mg/L+6-BA2.0 mg/L+coconut juice 10% got the best differentiation effect, differtntiation rate respectively:Da107 8.89%, Da107×NiuFangqie2.22%, Di D4 6.67%, Di D4xHong Qunlun4.44%.(3) The complete plantlets from the callus differentiation of thermo-sensitive genic male sterile lines of tomato bud formation and rootingwere got in the experiment.3 Microscopic observation result of thermo-sensitive gentic male sterile lines of tomato anther culture approach:During the thermo-sensitive gentic male sterile lines of tomato formation approch from, by parafilm cut, it was observed in mononuclear pollen grain,2-cellular period and multicellular ball, evenmorn embryonic callus and callus were seen.
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