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PRRS virus establishment of three different detection methods

Author: GuHaiYang
Tutor: LiLianRui；YangSu
School: Tarim University
Course: Biochemistry and Molecular Biology
Keywords: PRRS virus Nsp2 gene ORF7 gene Detect
CLC: S858.28
Type: Master's thesis
Year: 2011
Downloads: 192
Quote: 0
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Abstract


PRRS virus can cause sow abortion , premature birth, stillbirth , mummified piglets physical weakness and other symptoms , resulting in significant economic losses. In recent years, in our country there is a new highly pathogenic virus , in order to detect this new virus needs to establish a series of test conditions to meet the different detection methods . To this end we have established a RT-PCR, RT-LAMP and Real-Time PCR for the detection of these three conventional methods . In this study, the highly pathogenic PRRS virus as the research object , the view from the NCBI many highly pathogenic PRRS virus and normal pathogenic PRRS virus sequence analysis of highly pathogenic blue- ear disease and normal PRRS virus gene structure , selection Nsp2 ORF7 genes and genes as the main object of study, design and more pairs of primers and probes used for RT-PCR, RT-LAMP and Real-Time RT-PCR fluorescence method detection and analysis . The PRRS virus inoculated in Marc-145 cells were cultured cell lesions observed symptoms . The diseased cells after repeated freezing and thawing three times for virus subculture . Then take after repeated passaging of the virus culture medium , respectively RT-PCR, RT-LAMP , and Real-Time fluorescence PCR assay . After testing found that the highly pathogenic blue-ear disease and PRRS virus compared to traditional in Nsp2 lack of a continuous 87bp , while ORF7 is more conservative , the establishment of three kinds of test conditions to meet different methods to facilitate the PRRS testing.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Pig
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