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QTL Mapping for Grain Yield Associated Traits Using Introgression Lines in Maize

Author: ZhaoZuo
Tutor: ZhangZuXin；TaoYongSheng
School: Agricultural University of Hebei
Course: 作物育种学
Keywords: Corn Introgression lines Yield Quantitative trait loci
CLC: S513
Type: Master's thesis
Year: 2011
Downloads: 33
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Abstract


Introgression lines (Introgression Lines, ILs) backcrossing and molecular marker assisted selection to build a permanent mapping population , carried out the the QTL genetic analysis of the ideal material . Compared with traditional mapping populations , single segment introgression lines clear genetic background population genetic stability , high accuracy and sensitivity of the of QTL positioning and identification , accurate analysis of QTL genetic effects , etc. . Identification of maize production trait loci and contain favorable alleles Import Department , to provide reference and basis for the understanding of the genetic basis of yield traits and for maize inbreds molecular design . This study for the donor parent to QB80 and Qi319 , tuck 478 as the recurrent parent from BC1 backcross generations take at least 50 individuals of mixed pollen and tuck 478 consecutive backcrossing was BC4F1 , BC4F1 selfing form a the 240-300 a monoclonal BC 4 F 2 . The yield traits Ye478 exist differences in implementation directional selection in BC 4 F 2 monoclonal continuous selfing formation of 61 and 72 family groups Introgression Lines 2 generation . Through two years of field trials , yield and its use of complete composite interval mapping QTL (Quantitative Trait Locus, QTL) analysis . The major findings include : 10 traits , 55 QTLs , four environmental QB80 donor introgression lines population , were detected ; 10 traits were detected in Qi319 donor Introgression Line Population 49 QTLs. 18 QTLs were detected in the environment 2 and above . The QB80 tuck 478 genetic differences than Qi319 greater , QB80 donor groups introgression lines can be detected more QTLs for yield traits . The same trait in different environments to detect QTL mapping different traits QTL in the same region of chromosome positioning in the formation of multiple QTL -rich region in the same or close to the chromosomal region . QTL -rich region is available for reference for yield traits Cloning provide important chromosomal regions . QTL loci detected by the two groups having the consistency of less the two donor material containing different advantageous genetic loci . 3 . Import fragment containing niche due to the import of the Department and its related traits significantly be improved , these introgression lines for QTL polymerization improved Ye478 yield related traits .

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CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
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