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The use of low- dimensional structure of single DNA molecules in nature

Author: HuiDan
Tutor: WangKaiGe
School: Northwestern University
Course: Optics
Keywords: Nanopores Nanocolumn Micro-and nano -channel DNA molecules Kinetic properties
CLC: TB383.1
Type: Master's thesis
Year: 2011
Downloads: 50
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Abstract


In the life science and technology continues to move forward in the context of the relevant properties of DNA molecules gradually toward low-cost, more efficient and rapid detection direction. Currently, nanopore technology has been successfully applied to DNA sequencing work of r , while the nano -channel technology also shows the DNA molecule stretching and dynamic characteristics analysis of the great advantages of being more like nanostructures and more and more used in the detection of biological Oita f , which will help to achieve a single molecular level the effective manipulation of biological molecules , and thus more in-depth interpretation of the activities of life on the mystery of the cause of human health research has laid a solid theoretical foundation . The main contents include the following aspects : First, the hydrophobic PMMA surface to achieve the DNA molecule stretching, study the characteristics of the DNA molecule , and to provide an effective method of DNA stretching , preparing for the post- test in the solution , the external conditions so well prepared Secondly, in electrochemistry, spin coating methods such as copying a template prepared under PMMA nanorods , such as micro-and nano -channel structure , the use of such nanostructured surfaces to study the nature of DNA molecules , DNA molecules observed in its upper state of motion , functional analysis of these nano- structures on the mechanism of DNA molecules , as well as the forces of DNA molecules analyzed . Again , the use of AAO nanopore arrays in micro-current system detects nanoparticles through the situation , according to the ups and downs of the current situation analysis of nanoparticles through the properties. Finally, the DNA molecules AAO observed as a substrate on which the motion state , and the nano-columns , PMMA surface state of the substrate to compare a variety of different nano- structures were analyzed on DNA molecule.

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