Dissertation > Excellent graduate degree dissertation topics show

Pyruvate metabolism by increasing the flow of nodes to improve human-like collagen Ⅱ production

Author: ZhangGaoPing
Tutor: LuoYanE
School: Northwestern University
Course: Biochemical Engineering
Keywords: Pyruvate Like collagen Recombinant E. coli Response surface methodology Ferment
CLC: TQ936.2
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


This paper studies by recombinant Escherichia coli BL 21 fermentative production of human-like collagen protein phase Ⅱ expression of pyruvate added to improve the human-like collagen Ⅱ production. Response surface methodology and the use of the process of adding the effect of pyruvate class Ⅱ collagen production of human factors ( added amount of pyruvate , pyruvate phosphate buffer, adding time and concentration) for the shake flask experiments optimized. Finally, in a phosphate buffer concentration is 0.17mol / L of fermentation medium , adding the induction 8.23mmol / L pyruvate human collagen Ⅱ was obtained The yield was 0.29g / L, the experimental group compared to the control growth 11.54% , the cell OD60 ¨ 0 than the control group increased 28.72% . In shake flask on the basis of experimental data , with the bioreactor added amount of pyruvate , pyruvate Added two factors on human collagen type Ⅱ in yield were studied . The results showed that : can significantly improve the human-like collagen Ⅱ production of pyruvic acid dosage 15.84mmol / L, pyruvic acid were added to the induction of one hour . In this condition, the cell dry weight and protein production reached 69.6g / L and 11.87g / L.

Related Dissertations

  1. Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
  2. Study on Application of Non-phosphate Additive in Frozen Penaeus Vannamei and Mechanism of Water-holding,TS254.4
  3. Modification for PVA-Based Composite Packaging Material with Nano-SiO2 and Its Influence on Fresh Preservation Effect of Salted Duck Eggs,TS253.46
  4. Studies on Fermentation of Unconventional Protein Feeds and Their Application in Broiler Diets,S831.5
  5. Optimization of Extraction of Gastrodin from Rhizoma Gastrodia by Response Surface Methodology,R284.1
  6. Screening of Pigment-free Pullulan High Producing Microorganism and Research of Optimal Culture Media,TQ920
  7. Coagulation factor Ⅷ in Pichia pastoris expression,R554.1
  8. Rubber - cobalt - nickel- Preparation of adhesion promoters,TQ430.1
  9. Xining tomato endophytic colonization of Bacillus law and should promote effective research,S641.2
  10. Hypertrophic scars CD90, Ⅰ, Ⅳ collagen and TGF-β Ⅰ type , Ⅱ-type receptor expression correlation analysis,R622
  11. Pentamethylquercetin on diabetic nephropathy in rats and its mechanism,R285.5
  12. Low- diacetyl of Beer Yeast Mutants,TS262.5
  13. SCP-88 inhibited high glucose -induced rat mesangial cells extracellular matrix protein,R692.9
  14. The Research and Development of Monosodium Glutamate Production Line Based on PCS7 Control System,TP273
  15. Analysis on Expression Patterns and Prokaryotic Expression of Pyruvate Dehydrogenase Kinase Gene of Silkworms, Bombyx Mori,S881.24
  16. Expression and Significance of TGF-β1/Smad Signaling Pathway in Hepatic Fibrosis of Mice During the Middle Stage of Infection in Mice Infected by Echinococcus Multilocularis,R532.32
  17. Solid-liquid system under the white rot fungi biodegradation studies hexachlorobenzene,X172
  18. Microbial fermentation production of Coenzyme Q_ (10),TQ925.9
  19. Fermentation, Isolation, Purification and Structural Identification of Antimicrobial Substances Produced by Brevibacillus Brevis XDH,Q93
  20. Study on the Treatment of Potato Starch Wastewater and Method for COD Removal in Utilization Technique of Potato Starchy Residue,X703
  21. Primary Study on the Thermophiles from Pu’er Tea During Pile-Fermentation Process,TS272

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Protein ( PrP ) and the chemical processing industry > Chemical processing > Various proteins
© 2012 www.DissertationTopic.Net  Mobile