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The method of cottonseed protein extracted from cottonseed meal were studied, the byproduct of the extraction process of Raffinose separation and purification and drying process at the same time conducted research . Crushed cottonseed meal , first with an aqueous solution containing raffinose extraction, in order to avoid the impact of raffinose cottonseed protein subsequent separation and purification . The optimum water extraction conditions : The amount of water added is 20 times the raw material quality , the extraction time was 2 hours under the conditions of temperature of 40 ℃ , cottonseed sugar extraction rate was 98.7% . Plant cell walls made ??of cellulose and pectin , cottonseed cell wall is too hard , in order to improve the rate of extraction of cottonseed protein using cellulose complex enzyme cottonseed cell wall destruction , easy to extract the cells inside the protein . The water extracted cottonseed meal with cellulose enzymatic hydrolysis conditions were as follows : pH 5.0, temperature 50 ° C , enzymatic hydrolysis time was 2.0 hours , 0.4% of the amount of the enzyme . Soluble in water and then alkaline protease Alcalase 2 cottonseed protein hydrolyzate into peptides and amino acids , so as to improve the purposes of the cottonseed meal inside cottonseed protein extraction rate , which alkaline protease Alcalase 2 , best hydrolysis conditions : pH 8.5 , temperature 55 ° C , enzymatic hydrolysis time of 3.0 hours , 4.0% of the amount of the enzyme . At this point , cottonseed meal protein extraction rate of 86.4% . Raffinose extract byproduct aqueous solution after microfiltration membrane N100 impurity , the raffinose transmittance can be up to 98.7% , impurities interception rate of 18.9% ; then concentrated by nanofiltration membrane DLC election concentrated raffinose retention rate of 99.4% , the raffinose purity of 87.5% , dried raffinose dry powder products .
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