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Isolation and Identification of Dairy Cow Endometritis Pathogenic Bacteria and Bacteriostatic Experiment in Beijing Region

Author: ZhangDaiNa
Tutor: MaYuZhong；LiuYan
School: Agricultural University of Hebei
Course: Clinical Veterinary Medicine
Keywords: Beijing area Dairy cow Endometritis Isolation and identification of pathogens Bacteriostatic
CLC: S858.23
Type: Master's thesis
Year: 2011
Downloads: 135
Quote: 0
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Abstract


Cow endometritis cows common obstetric disease, can lead to reproductive failure caused great economic loss to the Dairy Industry. This test selected postpartum normal cows suffering from endometritis cows as an object of study, collecting the Beijing area dairy farm eight normal cows and 60 uterine endometritis cows uterine secretions, were isolated and identified the cause of endometritis The main pathogens. Bacteria isolated from 0-30 d postpartum normal cows uterus to the maximum number of 2-7d postpartum detection of bacteria. Isolated from the risk of uterine endometritis cows uterus pathogens as Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Enterococcus faecalis, Bacillus subtilis, which provides a further study of cow endometritis reference. In order to study the sensitivity of western medicine cow endometritis pathogens, the test antibiotics, medicine endometritis mainly pathogenic bacteria test. The results show that the strong inhibitory effect of ampicillin and ciprofloxacin against these pathogenic strains, such as penicillin, streptomycin poor inhibitory effect of these pathogens, but they are different patterns of resistance . Chinese medicine honeysuckle, forsythia, the Dandelion antibacterial better. This reasonable clinical medication to provide an important basis for the treatment of bovine endometritis. The test tube double dilution method, determination of the inhibitory effect of Chinese medicine cow endometritis pathogens. The results show that, significant inhibition of honeysuckle, forsythia, dandelions and other herbs, Chinese proprietary cow endometritis pathogens. By vitro antibacterial test selection effect good set of square used in clinical treatment of bovine endometritis. Vivo test, select 10 postpartum healthy cows as a normal control group, 20 ringleader endometritis cows suffering were randomly divided into a treatment group and the untreated group, and 3 respectively before treatment and after treatment, 5d, 7d jugular vein blood collection. Through the clinical pharmacodynamics test, determination of superoxide dismutase (SOD), malondialdehyde (MDA), to observe the clinical effect of traditional Chinese medicine prescription treatment cows uterus endometritis. The results show that: the Chinese proprietary improve cow serum SOD, GSH-Px activity, lower serum MDA, enhancing the body's antioxidant capacity, weakening the body's level of lipid peroxidation and increase serum IgG, IgA content, and enhance endometritis the cows immune function. Conclusion: Antibiotics have some effect separation pathogens, but different patterns of resistance, the Chinese proprietary treatment of bovine endometritis good results.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Cow
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