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Numerical Classification and Fingerprint Analysis of Chaenomeles

Author: YuXiaoLiang
Tutor: WangJianHua
School: Shandong Agricultural University
Course: Crop Cultivation and Farming System
Keywords: Papaya Breed Germplasm Classification number Fingerprints
CLC: S661.6
Type: Master's thesis
Year: 2011
Downloads: 61
Quote: 2
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Abstract


Through this experiment, Chaenomeles (Chaenomeles) germplasm resources survey, the choice of 22 fruits and seeds on morphological traits, including 22 of 90 papaya cultivars germplasm resources of quantitative taxonomic clustering analysis. Cultivar clustering results showed that: Papaya is a source of germplasm varieties can be divided into Japanese quince germline (Ⅰ), C.speciosa germline (Ⅱ), hairy leaves papaya germline (Ⅲ) and Tibet papaya (Ⅳ) 4 a characterization group. Chaenomeles Germplasm Resources clustering results showed that: the classification results of papaya germplasm resources and cultivars classification results are basically consistent; germplasm papaya leaves hair more germline and C.speciosa germline can be divided into four, and two each cultivar groups. Principal component analysis showed that: 22 traits for the six principal components integrated, the main component cultivars cumulative contribution rate of 88.323%, 70.224% germplasm resources. Integrated cluster analysis and principal component analysis of the results combined with practical application, the authors believe is through papaya fruit characteristics of the species should be classified provenance (germ line) as a standard, fruit shape index and seed morphology as an important indicator; fruit shaped as two classification criteria; fruit ribs as three classification criteria. 74 parts of a representative selection of papaya germplasm resources of its chlorogenic acid, oleanolic acid, ursolic acid and total flavonoids were determined. Results Chaenomeles not detected in chlorogenic acid, oleanolic acid and ursolic acid contents were 0.106 -0.108 mg · g -1 ,0.694-0 .840 mg · g - 1 , total flavonoids was 16.135-28.182 mg · g -1 ; Japanese quince chlorogenic acid content of 0.117-1.694 mg · g -1 , Qi oleanolic acid and ursolic acid contents were 0.441-0.911 mg · g -1 ,0.366-0 .487 mg · g -1 , total flavonoids was 16.424-19.804 mg · g -1 ; C.speciosa chlorogenic acid content of 0.096-2.746 mg · g -1 , oleanolic acid and ursolic acid contents were 0.360-1.836 mg · g -1 ,0.110-1 .604 mg · g -1 , total flavonoids was 6.661-40.839 mg · g -1 ; hair Papaya leaf chlorogenic acid content of 0.297-4.017 mg · g -1 , oleanolic acid and ursolic acid contents were 0.311-1.520 mg · g -1 , 0.031-1.345 mg · g -1 , total flavonoids was 8.428-39.517 mg · g -1 . C.speciosa papaya 39,44,46,48 13 and hairy leaves, rich in chlorogenic acid content reaches 3 mg · g -1 -4 mg · g -1 ; papaya leaves hair 53 and C.speciosa 8,23, oleanolic acid rich reached 1.5 mg · g -1 above; hairy leaves of papaya C.speciosa 8,24 48 and ursolic acid, etc. higher 1.3 mg · g -1 above; higher levels of flavonoids hairy leaves of papaya papaya germplasm C.speciosa 35 and 15, the total flavonoid content of up to 40 mg · g -1 < / sup>. In the morphological classification based on the genus papaya fruit components were HPLC fingerprint studies, the establishment of papaya fruit genus germline HPLC fingerprint; papaya on 90 germplasm resources were HPLC fingerprint identification; and 22 cultivars and 90 Papaya germplasm fingerprinting cluster analysis. Papaya leaves, represented by gross inspection, Anhui, Shandong, Hubei, Yunnan and other landowners production papaya HPCL fingerprint differences. By papaya fruit morphology and composition are two aspects of the research are combined to create a papaya germplasm resources database; improved papaya genus classification system; provides papaya identification, quality evaluation and quality standards theory. Medicinal Plant Resources of Chaenomeles made edible classified management, both to ease the classification named the chaos, but also to meet the urgent needs of the actual development, resource development papaya basically solved the theoretical problem.

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CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Pome > Papaya
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