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Calcineurin B-like protein (calcineurin B-like proteins, CBLs) recently identified a class of plant-specific Ca 2 sensor, its protein interaction CIPK (CBL-interacting protein kinase) play an important role in the plant specific Growth and answering Stress process. Studies have shown that, CIPK widely involved in the response of plants to high salinity, drought and low temperature abiotic stress. For CIPK mainly in Arabidopsis, rice fine on a small number of model plants, little fruit trees aspects. For many reasons, the less and less arable land used to, at the same time, due to climate change worldwide, fruit tree planting area and scope will seriously be affected. Mining the apple the CIPK genes and functional studies, not only has an important theoretical significance, but also with a wide range of applications, antiretroviral gene breeding potential candidate genes for fruit trees. Cloned from apple a of CIPK gene MdCIPK6, and analysis of the characteristics of its gene structure, expression specificity and its function. The major research work and the results are as follows: using the RACE method, cloned MdCIPK6 full-length cDNA, and the total length is 1565bp, wherein the ORF region of 1299bp encodes a polypeptide containing 433 amino acids, the prediction of the PI of 8.98, a molecular weight of 48.50 kDa. NAF conservative structure protein structure with the CIPK common N-terminal kinase domain and the C-terminal domain. The semi-quantitative RT-PCR results show that it is constitutively expressed in various tissues and organs, and the relatively low amount of expression in the root. However, with NaCl and ABA treatments Gala rooting plantlets found MdCIPK6 significantly by inducible expression in roots. In addition, in the abiotic stress stress hormones as well as the exogenous Ca 2 under treatment, can significantly induce its expression. MdCIPK6 gene may be an important role in the stress response of the resilience. Yeast two-hybrid test showed that, MdCIPK6 and Arabidopsis AtCBL4 (AtSOS3) protein interactions; complementary function test results show that the MdCIPK6, and MdCIPK6T175D able complementary Arabidopsis sos2 ??for mutations body Salt aspects function. Overexpression in wild-type Arabidopsis MdCIPK6, and MdCIPK6T175D able to significantly improve stress resistance. The description MdCIPK6 stress resistance through the SOS pathway regulation. 4. Identification MdCIPK6 gene, Apple stress resistance (such as salt, drought and cold resistant, etc.) in the function carried Apple transgenic research, the results show that, compared with the wild-type and antisense transgenic lines, overexpression MdCIPK6 and MdCIPK6T175D able to significantly improve stress resistance of Apple, showed that MdCIPK6 Apple stress resistance positive regulatory factors. To further identification MdCIPK6 gene function in crops, tomato overexpression the MdCIPK6 genes, the results showed that the transgenic tomato germination, seedling and stress resistance into seedling stage were significantly increased, indicating MdCIPK6 biological function in different plants between conservative.
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