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Common wheat (Triticum aestivum L.) is one of the world's most important and oldest food crops . Bread , noodles , dumplings and other foods made ??with wheat flour is the traditional staple food of the people of our country . Wheat flour whiteness is an important indicator of the quality of the merchandise flour , grain containing polyphenol oxidase (polyphenol oxidase, PPO) is the main reason for browning caused by the color of dough (film ) . Therefore , in-depth study of wheat grain PPO activity and molecular markers positioning has an important significance for the genetic improvement of wheat flour whiteness . The Anther Culture No. 3 Yumai 57 by crossing double haploid (Doubled Haploid, DH) groups for mapping population , Measurement of Wheat PPO activity in the environment , and the use of mixed linear model QTL mapping software Icimapping2.2, PPO activity analysis, to lay the foundation for PPO activity of gene cloning and molecular marker-assisted selection . The main findings are as follows : three environmental conditions ( 2009 normal water and fertilizer , nitrogen fertilizer in 2009 , the 2009 rainfed Tian ) DH population of 168 lines planted there are obvious differences , their parents PPO activity groups PPO activity meets the absolute value of the normal distribution , the value of skewness and kurtosis values ??less than 1.0 indicated that the population for QTL analysis . 2 . 315 SSR molecular marker loci anchor the in wheat 21 staining . Map of full-length 2141.7cM, the average genetic distance between the two markers 7.02cM, forming 24 linkage groups . Each linkage group 3 to 24 sites , found some mark -rich region between these regions in different mapping populations is highly polymorphic favor QTL mapping . Control wheat grain PPO activity detected 13 sites located in chromosome 8 (2A, 2D, 3D, 5A, 5B, 6A, 6B and 6D) , which the seven QTLs genetic contribution rate (more than 10 %) is the major gene locus , the remaining six QTLs genetic contribution is small ( less than 10% ) , genus loci minor .
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