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Wheat ERF transcription factor gene cloning and functional analysis

Author: GuanErZuo
Tutor: LiYing；ZuoRuiMing
School: Shenyang Normal
Course: Biochemistry and Molecular Biology
Keywords: Wheat ERF transcription factor Stress Gene Expression Subcellular localization
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 116
Quote: 0
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Abstract


Plant growth and development are often subject to the impact of Stress , Stress including biological stress and abiotic stress . The stress on the plant growth and crop and quality caused a serious impact. Stress occurs in the plant stress signal sensing and passed through activation of transcription factors in response to start the expression of stress tolerance genes , the plant body of a series of physiological and biochemical reactions of the plant to a certain extent, can withstand the stress on the injury. Numerous studies show that plants AP2/EREBP transcription factors outside the plant response to stress and increase the resistance of plants plays an extremely important role . Therefore research ERF transcription factor mechanism of action has important theoretical and practical significance . Constructed by homologous cloning from a non- pro and small types of infection -induced wheat cDNA library ( by the Chinese Academy of Agricultural Sciences Institute of Plant Protection wheat crop diseases research group build ) has been two wheat ERF transcription factor genes in all length cDNA clone , named TaERF4, TaERF5. According to its predicted amino acid sequence analysis showed that they contain a conserved AP2 domain , and published ERF subfamily members have high homology . Homology analysis showed that , TaERF4, TaERF5 typical ERF subfamily members of the close proximity of the evolutionary tree , TaERF4 / 5 may be a new AP2/EREBP transcription factor genes. Semi- quantitative RT-PCR analysis showed that , TaERF5 made ​​for ABA ABA response , TaERF4, TaERF5 MeJA made ​​for methyl jasmonate response in the treated , the transcriptional levels ; at low temperature , drought and high salt treatment , the expression of the two genes were significantly increased. Using the biolistic method of TaERF4 / 5 subcellular localization were studied, results show TaERF4 / 5 with localization capabilities. Through the above experiments show , TaERF4 / 5 due to transcription factor genes have AP2/EREBP basic features . TaERF4 / 5 gene by cold , drought and high salt abiotic stress induction . These findings wheat molecular breeding for resistance provides a theoretical basis , has important practical significance.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
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