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Poria Cocos Protoplast Fusion and Fermentation Dynamics Research

Author: LiuYuZuo
Tutor: YangShengYu
School: Henan University
Course: Microbiology
Keywords: Poria Protoplast Polysaccharide Dynamics Liquid fermentation
CLC: S567.32
Type: Master's thesis
Year: 2011
Downloads: 38
Quote: 1
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Abstract


The Poria part of the fungus door, is a medicinal and edible fungus. The Pachymaran Poria a class with a wide range of physiologically active substances, has high development value and a good prospect. In order to get a good strain, strain polysaccharide production, improve the efficiency of fermentation, we protoplast fusion breeding and fruiting culture of the original strain HD09-10. Liquid fermentation culture Poria mycelium the extract Pachymaran study of liquid fermentation conditions and rules. By single factor experiment, the incubation time of the protoplast preparation, enzyme concentration, reaction temperature, hydrolysis time, enzymatic hydrolysis pH, steady infiltration etc. conditions. Obtained by orthogonal experiments the optimum conditions for protoplast formation and regeneration: cell age for two liquid cultured for 4 days, 3% of the enzyme concentration (snail enzyme with dissolved wall enzyme ratio of 2:1), enzymatic temperature of 36 ° C, enzymatic time 2.5 h, enzymatic pH 6.5, 0.6 mol / L osmotic stabilizer. Screening to get of higher production the Pachymaran content faster growth vigorous mycelium strains by protoplast fusion. Culture of the parent and fusants fruiting body. From medium composition, lighting, culture temperature, number of passages several factors to test the best conditions of the fruiting body of Poria strains formed under laboratory conditions. The experimental results show that, Poria strains cultured in PDA medium temperature 27 ℃, 50% of the light intensity, incubation time 18 to 30 d, passage number 5 to 7 generation more prone fruiting bodies. In Poria liquid fermentation by culture conditions and optimization of the medium, to explore different ventilation conditions Poria optimal culture environment, in order to facilitate the mycelial growth and polysaccharide secretion, and analysis of the polysaccharide molecular weight distribution. The results showed that adding 4% of the olive oil in a the Poria liquid fermentation speed of 120 rpm at 26 ℃, conditions, not only conducive to the growth of mycelium, but also conducive to the secretion of the polysaccharide. Preferred in the fermenter culture, aeration rate of 2 vvm, and 0.22 mg / mL of the extracellular polysaccharide can be obtained. Using optimized process conditions, the use of Origin software for data processing, Poria fermentation process dynamics mathematical models and model parameters simultaneously compare the experimental data with the model verification. In medium volume 3L/5L, impeller speed 120 r / min, inoculum size 8% and 2 vvm ventilation conditions, fermentation get exopolysaccharide concentrations up to 37.54 mg / dL, the model calculations and experimental results fit the curve consistent, good model reaction Poria fungus bacteria and extracellular polysaccharide fermentation process and its kinetic mechanism.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Fungus > Poria
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