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Objective: To study the salivary gland mucoepidermoid carcinoma of HIF-1α, Glut-1 expression, analysis of the relationship between the level of both expression and MEC different clinical staging and pathological grading, and the correlation between the two; explore in MEC diagnostic and prognostic significance, whereby open up new ways to find new therapeutic targets for gene therapy of the salivary gland mucoepidermoid carcinoma. Method: specimens taken from the First Hospital of Shanxi Medical Oral and Maxillofacial Surgery from 2000 to 2009 were treated salivary mucoepidermoid cancer archived paraffin blocks of 60 cases, and normal salivary gland tissue of 20 cases. Using semi-quantitative immunohistochemical methods to detect the MEC tumor tissue HIF-1α and Glut-1 expression, application of Envision PV-6000 Power Vision TM two-step staining. This study SPSS13.0 software for statistical analysis, taking P lt; 0.05 as statistically significant. HIF-1α, Glut-1 protein expression in the MEC various clinical parameters between comparison using χ2 test; the two indicators expression using Spearman rank correlation analysis. Results: 1.HIF-1α MEC specimens expressed mainly in the nucleus, and also a small amount of expression in the cytoplasm. High expression levels in the MEC (65%) was significantly higher than that in normal salivary gland tissue (0%), P lt; 0.05 was statistically significant, suggesting that HIF-1α involved in the development of the MEC. Its expression levels and the patient's gender (P = 0.251), age (P = 0.244) and lymph node metastasis (P = 0.25), the difference was no statistical significance (P gt; 0.05). Compared with the degree of tumor differentiation, size and clinical staging difference was statistically significant (P lt; 0.05); high expression rates were 35% in the high school poorly differentiated, 77.8%, 81.8%; T1 T2 and T3 T4 high expression rates were 44.8%, 83.9%; clinical stage Ⅰ Ⅱ Ⅲ Ⅳ high expression rates were 48.5%, 85.2%; This shows that the higher the histological grade, the higher the degree of malignancy, tumor growth and invasion more quickly, and also a higher degree of hypoxia, a more obvious expression of HIF-1α. 2.Glut-1 MEC specimens mainly expressed in the cell membrane, and a small amount of expression in the cytoplasm. High expression levels in the MEC (72%) was significantly higher than that in normal salivary gland tissue (0%), P lt; 0.05 was statistically significant, indicating that the Glut-1 related to the development of the MEC. Its expression levels and patient gender (P = 0.714), age (P = 0.485) and lymph node metastasis (P = 1.0), the difference was not statistically significance (P gt; 0.05). Compared with the degree of tumor differentiation, size and clinical staging difference was statistically significant (P lt; 0.05); high expression rates were 50% in the high school poorly differentiated, 77.8%, 86.4%; T1 T2 and T3 T4 high expression rates were 55.2%, 87.1%; clinical stage Ⅰ Ⅱ Ⅲ Ⅳ high expression rates were 57.6%, 88.9%; shows that the higher the pathological grading, the larger the tumor, the degree of malignancy of the more , the faster the growth rate, the oxygen demand may decline in oxygen concentration, rely on glycolysis to obtain energy to improve hypoxia in this hypoxic state, resulting in the enhancement of Glut-1 expression. 3.HIF-1α and Glut-1 expression in the MEC was positively correlated (rs = 0.463, P lt; 0.05). Tip HIF-1α intensity of Glut-1 over-expression, may by regulation to meet the needs of the energy metabolism of sugar consumption increases in salivary mucoepidermoid carcinoma growth process. Conclusion: HIF-1α and Glut-1 expression and the degree of tumor differentiation, size and clinical stage are closely related, and can be used as a predictor of MEC prognosis.
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