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Objective: To study the impact of intervention after pilocarpine epileptic hippocampal tissue , Cx43, Cx32 expression changes , Health and carbenoxolone , explore of Cx43 of Cx32 role in the pathogenesis of epilepsy and raw stomach ketone antiepileptic mechanism . Methods: SD rats were randomly divided into control group , the pilocarpine model group , Health and carbenoxolone intervention group 3 group . Application of lithium chloride - horses Lucas products intraperitoneal injection method for the preparation of epilepsy model . Model group , raw stomach ketone intervention group based on the 3, 6, 12 and 24 hours after the seizures , 3 days and 7 days divided into six subgroups . Health the carbenoxolone intervention group in the injection of pilocarpine 1 hour before intraperitoneal injection of 20mg/kg standard . The control group without any treatment , conventional breeding . Using the the Racine classification method evaluation of epileptic rats attack level and record the onset of the incubation period ; the HE staining observed morphological changes in hippocampal organization ; hippocampal Cx43 and Cx32 expression using immunohistochemical methods to detect . Results: control rats without seizures , the model group and the intervention group seizures incubation period was no significant difference ( P gt ; 0.05) . HE staining showed that the model group, hippocampal region began to appear in the six hours after epileptic cell shrinkage, drop out, especially in the CA1, CA3 region and dentate gyrus , the seventh day, the most severe epileptic ; intervention group also experienced this phenomenon However, the extent of neuronal loss compared with the model group . Compared with the control group , the model group and raw stomach ketone intervention group hippocampus of Cx32 expression levels of Cx43 increased significantly ( P lt; 0.05 ) , 24 hours after the epileptogenic peaked , after gradually reduce ; within 24 hours after the epileptogenic model group and the intervention group Cx32, Cx43 expression was no significant difference (P gt; 0.05), but 3 days and 7 days group , model group and the intervention group expression differences ( P lt; 0.05 ) . Conclusion : electrical synapses of Cx32 and Cx32 constitute between neurons involved in the development of epilepsy ; seizures , Cx43 expression increased the result is both epilepsy occurs , they may be a contributing factor of epilepsy development ; 3. acute seizures of Health carbenoxolone did not exhibit obvious protective and (or ) a therapeutic effect , the stationary period showed a significant neuroprotective effect .
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