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A Objective: To investigate the angiotensin Ⅱ (Ang Ⅱ) on wound healing in the proliferation of fibroblasts and different concentrations of Ang II wounds role differences, designed to provide for future clinical experiments reference. Two methods: a model making: 10 male SD rats, weighing 175 2 00g, Department of Physiology, Shanxi Medical Animal Center. Each four wounds, belonging to four groups (NS group, the low-dose group, middle dose group, high dose group), ie 10 wounds in each group. Injury that day, minus the back of the long-haired, iodophor disinfection. Pentobarbital doing anesthetized by intraperitoneal injection animals fixed with professional puncher punch (hole 1.5 cm × 1.5 cm), resulting in the back of the animal on each side 2 of about 1.5 cm × 1.5 cm full-thickness skin wounds. The 2 wounds medication: NS group, wound treatment drug for saline; low-dose group, wound therapy for 1 × 10 -9 mol / ml Ang Ⅱ solution; middle dose group, wound therapy 1 × 10 -8 mol / ml Ang Ⅱ solution; high-dose group, wound therapy for 1 × 10 -7 mol / ml Ang Ⅱ solution. Each group the animal wound with full treatment of drug the gauze wet dressing, plus sterile moisturizing film covering the wound dressing change every day, until the first five days. Animals in each group were individually fed, free drinking water, eating. Detection indicators and methods: observation of the animals back four wounds, were hurt after 5 days with a transparent film depiction meter plus the use MIG2000 software calculated the wound area; take 4 about 1.0 cm × 1.0 cm wound tissue of the back of the animal, do organizations uniform pulp, centrifuged supernatant into single cell suspension. PI staining, 10,000 cells in S phase (DNA synthesis phase) as the proportion of fiber cells by flow cytometry. 4 statistical methods: data with SPSS13.0 measurement data were expressed as mean ± standard deviation ((x | -) ± S) data analysis designed using a repeated measures analysis of variance test P lt; 0.05 as significant difference. Three results: 1 Wound area: 5 days after injury, the the groups wound area high dose group (1 × 10 -7 mol / mL) minimum area of ??202.6 ± 0.2 mm wound 2 ; NS group (saline) was 215.53 ± 0.28 mm 2 , between the two groups wound healing rate significantly poor (P lt; 0.05). The remaining two groups wound area were: low dose group 209.56 ± 0.29 mm the 2 , the middle dose group 207.03 ± 0.26 mm 2 ; wound area among the groups pairwise comparisons are significant difference (P lt; 0.05). Determination of cell cycle of wound tissue results: application of Ang Ⅱ group wound cells in S phase percentage is higher than the NS control group, a statistically significant (P lt; 0.05); different concentrations of Ang Ⅱ group, the percentage of cells in S phase differences are also statistics significance (P lt; 0.05). , With a higher drug concentration, an increase in the proportion of S-phase cells. Four Conclusion: Topical application of angiotensin II on systemic vascular contraction had no significant effect, it is safe and feasible. 2 local angiotensin II on the mouse full-thickness skin defect wound healing promote, may be related to local angiotensin II can accelerate wound organized into fiber cell differentiation, proliferation. 3 local angiotensin II can accelerate wound healing. 4 Topical application of angiotensin II treated mice full-thickness skin wounds, a concentration of 1 × 10 -7 mol / mL when the treatment effect, its efficacy dose - bio-effect relationship exists.
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