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Fluorescent probe method to study the mechanism of action of the drug and biological macromolecules and their determination

Author: HuangJuQin
Tutor: WangHuaiYou
School: Shandong Normal University
Course: Analytical Chemistry
Keywords: Bovine serum albumin Fluorescent probe Fluorescence Quenching Streptomycin Quercetin Abscisic acid
CLC: R346
Type: Master's thesis
Year: 2011
Downloads: 118
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Abstract

The mechanism and the mechanism of the binding between the drug and the biological macromolecule is an important component in the field of pharmaceutical science. Binding site and the mechanism of action of the drug in the human body has a direct impact on the therapeutic effect of the drugs on the disease. Therefore, the study of drugs and biological macromolecules in the the simulated human environment under the conditions of the binding site and mechanism of action is very necessary, can also ensure the safety of people's medication, reasonable and effective. Protein is important macromolecules in vivo, it can combine with the compounds of many endogenous or exogenous, and having a function of storage and transhipment. Drugs into the body generally has to go through the storage and transport of the plasma reaches the receptor site of the pharmacological effects of serum albumin and drug binding mechanism of action has important theoretical and practical significance. Currently, the method used to characterize protein - drug molecules binding fluorescent probe technique has been widely used because of its high sensitivity, good selectivity, easy. Fluorescent probe method is the use of the added role of drugs and probe molecules such that the fluorescence of the biological macromolecules or probe molecules changes to study the mechanism of action between the drug molecules and biological macromolecules, thereby further understand the role of the drug molecule in the human body mechanism. In this thesis, using of Fluorescent Probe drug molecules with bovine serum albumin binding site and mechanism, so as to further the understanding and the mechanism of action of the study drug in the body and the way to play a pharmacodynamics. The thesis is divided into five chapters chapter summarizes the interaction between drug molecules and serum albumin Research and research significance. Briefly describes the structure and physicochemical properties of the protein, followed by small drug molecules and serum albumin interaction research methods, content and research developments commenced comments. The second chapter of Evans blue (EB, Evans blue) and streptomycin (SM, streptomycin) bovine serum albumin competitive reaction mechanism using fluorescence spectroscopy and ultraviolet spectroscopy. Evans Blue capable of quenching the fluorescence of bovine serum albumin static quenching occurs. However, when added streptomycin, bovine serum albumin fluorescence recovery. This shows that the competitive reaction of EB and streptomycin BSA. Obtained the binding constants of bovine serum albumin and streptomycin the 6.52 × 10 ^{6 L / moL. Chapter fluorescence spectroscopy to study the interaction of bovine serum albumin and ABA. Abscisic acid and Bovine Serum Albumin bovine serum albumin fluorescence quenching occurs. According to the Stern-Volmer equation and fluorescence lifetime study the types and mechanisms of fluorescence quenching. Quenching mechanism is static quenching, abscisic acid and bovine serum albumin to form a stable complex. Synchronous fluorescence spectroscopy to determine the influence of abscisic acid on the micro-structure of the bovine serum albumin. Experiments seeking to forget the abscisic acid and bovine serum albumin binding constants and binding points K = 6.38 × 10 ^{4 L.mol ^{-1 , n = 1.452. Chapter fluorescent probe screening evaluation of the antioxidant capacity of the the natural antioxidant Pharmacy quercetin, 1, 3 - diphenyl-isobenzofuran (DPBF) as a fluorescent probe to study the antioxidant drug quercetin elements of singlet oxygen scavenging, discussed its mechanism of action, and experimental interfering substances. Chapter fluorescence quenching method abscisic acid in the determination of plant.}}}

Fluorescent probe method to study the mechanism of action of the drug and biological macromolecules and their determination

Abstract

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