Dissertation > Excellent graduate degree dissertation topics show

Interference Hes1 gene on bone marrow stromal cells into cholinergic neurons pre- impact

Author: LongZhiJing
Tutor: WangChunFang
School: Shanxi Medical
Course: Developmental Biology
Keywords: Bone marrow stromal cells Gene expression Hes1 Mash1 RNAi BMSCs Cholinergic neurons Cell Differentiation Real-time PCR
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract


Objective: To explore the expression of Mash1 after the inhibition of Hes1 gene inBMSCs.Methods: BMSCs isolated from bone marrow were cultured in vitro. The morphologicfeature and growth status of BMSCs and differentiated BMSCs were observed by invertmicroscope. The expression of Mash1 was analyzed by real-time quantitative PCR afterthe inhibition of Hes1 gene in BMSCs.Results: The concentration of Hes1 siRNA were selected for 200nM, 100nM, 50nMand 25nM. The expression of Hes1 was low-decreased in comparison to control group.The level of Hes1 at the concentration of 200nM in interfered BMSCs was 0.036-fold ofthat in control BMSCs (P<0.05)，and the level of Hes1 at the concentration of 50nM ininterfered BMSCs was 0.076-fold of that in control BMSCs (P<0.05), and they werebetter than the others. The expression of Mash1 gene was increased after the inhibitionof Hes1 gene with a dose-dependent effect.Conclusion: Hes1 can down-regulate the expression of Mash1 gene in BMSCs. Objective: To discuss the mechanism of cholinergic neurons differentiate induced frombone marrow stromal cells in vitro by analysising the impact of interfering expression ofthe gene Hes1 to the expression of the gene Sept-9 and Stmn1.Methods: The third generation of bone marrow stromal cells，seeded in polylysinecoated6-well culture plate，was induced to cholinergic neurons differentiation byadding the inducible factor RA, SHH and bFGF in the culture medium. To analysis theexpression of the gene Sept-9 and Stmn1 of interfering the gene Hes1 in the process ofBMSCs induced to cholinergic neurons differentiation by real time and quantitativePCR.Results: The expression of the gene Hes1 increased, after alone induced; Theexpression of the gene Hes1 increased significantly in the induction and differentiationgroup after interfered. The expression of the gene Sept-9 increased, after siRNA (50nM)interfered. There was no difference the expression of the gene Sept-9 compared with thecontrol group, when the BMSCs of P3 was alone induced in vitro. The expression of thegene Sept-9 increased significantly, when the BMSCs of P3 was induced after the geneHes1 was interfered. The expression of the gene Stmn1 increased significantly, whenthe gene Hes1 was interfered by siRNA (50nM); The expression of the gene Stmn1increased slightly, when the BMSCs of P3 was alone induced; The expression of thegene Stmn1 increased significantly, when the BMSCs of P3 was induced after the geneHes1 was interfered, and the increased range was among the interfered group andinduced group .Conclusion: It was very important for the expression of the gene Sept-9, Stmn1 tointerfer the expression level of the gene Hes1.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  3. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  4. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  5. The Detection of the Virulence-related Gene from Vibrio Alginolyticus and the Study on Preservation Methods,S943
  6. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4
  7. Study on Synthesis and Physiological Function of N-carbamoyl-glutamate,R914
  8. Control of Verticillium Wilt Disease of Cotton Plants with the Application of a Bio-Organic Fertilizer and Its Microbiologecal Mechanism in Rhizosphere,S144.1
  9. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  10. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  11. Study on Roles of Diacylglycerol Kinase Gene Family in Rice under Xylanase and NaCl Treatments by Transient RNA Interference System,S511
  12. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  13. Study of Chicken Δ~6 Fatty Acid Desaturase Gene’s Promotor Region Polymorphism and Gene Expression,S831
  14. Analysing Sequence Characters of ADSL and PurH Gene and Correlation between Genes Expression and IMP Content in Duck,S834
  15. Study on Characteristics、Cryopreservation and Gene Transfection of Goat Bone Marrow Mesenchymal Stem Cells,S827
  16. The Expression of CPV-2 VP2 Gene in SF9 and Development of Indirect Elisa for Serum Antibodys,S852.65
  17. Construction and Identification of Recombinant Lentivirus Expressing shRNA Targeting to ORF1 and ORF7 of Porcine Reproductive and Respiratory Syndrome Virus Genomes,S852.65
  18. Establishing and Applying a Rapid Detection System of Yersinia Enterocolitica,TS207.4
  19. An Application of Real-Time PCR in the Study of Biological Control of Tobacco Wilt Disease,S435.72
  20. Comparison of Gene Expression Data Cluster Methods and Gene Network Construction for Phytophthora Sojae Genes,S435.651
  21. Cloning and Characterization of Prodh Gene cDNA from Leptinotarsa Decemlineata (Say) and Bacterial Expression of dsRNA,S435.32

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile