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The Research and Application of Molecular Imprinting- Electrochemiluminescence Sensor

Author: QiWenJing
Tutor: SongQiJun
School: Jiangnan University
Course: Analytical Chemistry
Keywords: ECL Iridium Complexes Molecular imprinting technique Sensor L-tryptophan
CLC: TP212.1
Type: Master's thesis
Year: 2011
Downloads: 126
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Abstract


ECL (Electrogenerated Chemiluminescence, ECL) is directly or indirectly caused by the electrochemical reaction of the chemical luminescence phenomenon, and is the product of the combination of electrochemical and chemiluminescence. In ECL analysis study, the most widely used luminescence reagent ruthenium bipyridine and its derivatives. However, due to the limitation of the method itself, this reagent itself when applied will be continuously consumed, which also led to the analysis cost becomes high. The study found that the metal iridium complexes in the ground state, excited state redox potential and similar ruthenium bipyridine, and has a water-insoluble, therefore the the metal iridium complexes immobilized analysis applications is of great significance. Pure ECL modified electrode has high sensitivity in the analysis and detection, but poor selectivity. The molecular imprinting technique (Molecular imprinting technique, abbreviated as MIT), also known as molecular recognition, appeared in recent years, a process for preparing a target molecule having a specific selective recognition polymer technology. In this context, the molecular imprinting technique is applied to electroluminescent chemiluminescence analysis, the use of molecularly imprinted polymer specific recognition of the target molecule adsorption capture ability of the substance to be measured selectively separated from the complex system detecting, from the fundamental solution to the problem ECL sensor poor selectivity. Specific work as follows: 1. Introduced the principle characteristics of the ECL, research progress, experimental devices, focusing on the metal complexes immobilized. Through the literature review, an overview of the aspects of the research progress of molecular imprinting technique, the analysis of the characteristics, the existing problems and the prospects, and focuses on the preparation and application of molecularly imprinted electrochemical sensors. 2. Dispensing prepared by the physical and chemical properties of a molecularly imprinted electrochemical sensor performance, work sol - gel polymeric materials, L-tryptophan as a template molecule a molecular imprinted polymers were prepared using stable , good selectivity, wide linear range and low detection limit of molecularly imprinted electrochemical sensor, and the sensor by infrared spectroscopy and scanning electron microscopy were characterized. Through optimization of the conditions of the sol - gel preparation conditions, the template molecule removal time, and the template molecule and enrichment experiment, so that the sensor in a complex system to achieve a good selectivity, the linear detection range of 1.0 × 10 -9 ~ 1.0 × 10 -5 mol / L, the detection limit of 1.0 × 10 -12 mol / L. Prepared a carbon nanotube / polyvinyl alcohol / (pq) 2 the Ir (N-phMA)] modified electrode, and for the first time prepared ECL molecular imprinting technique combined with sensors, multi-walled carbon nanotube / polyvinyl alcohol / (pq) 2 Ir (N-phMA) modified electrode surface the secondary modified sol - gel molecularly imprinted polymers prepared by molecular imprinting - Electroluminescent chemiluminescence (MIT-ECL) sensor. In this paper, the introduction of polyvinyl alcohol (PVA) as a dispersing agent dispersed carbon nanotubes, and dispensing Preparation of multi-walled carbon nanotube / polyvinyl alcohol / (pq) 2 Ir (N- phMA) modified electrode. Sol - gel polymeric materials, L-tryptophan as a template molecules prepared molecularly imprinted polymers. Experiment by the amount of carbon nanotubes, the concentration of the iridium complex, the amount of the modifying agent, and the acidity and other conditions to be optimized so that the modified electrode to achieve optimum luminous efficiency. At the same time, the study of L-tryptophan as a template molecule, molecular imprinted polymer dosage, accumulation time and conditions of acidity to optimize good selectivity of the sensor in a complex system, a wide linear range (5.0 × 10 -14 1 .0 × 10 -10 mol / L) and low detection limit (1.0 × 10 < sup> -16 mol / L). Finally, using the sensor with traditional high performance liquid chromatography of L-tryptophan in the human serum for the analysis and detection, and achieved good results. Preliminary exploration of the ionic liquid and tris (2 - phenylpyridine) combined iridium complex in the electrically induced chemiluminescence in the application, preparation of 1 - iso-octyl-2 ,3 - dimethyl-imidazol-hexafluoro phosphate ion liquid as dispersing agent, a binder, tris (2 - phenylpyridine) iridium as a modified electrode of the light-emitting substance, experiments on the amount of the ionic liquids, the concentration of the complex, the system of acidity and the amount of modifying agent optimization, the modified electrode coreactant preferably ECL response.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Physical sensors
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