Dissertation > Excellent graduate degree dissertation topics show

Expression Analysis of Storage Protein Avenin-like B Gene Promoter from Wheat (Triticum Aestivum)

Author: SunYangLiu
Tutor: HeGuangYuan
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: Wheat Avenin-like b gene Promoter Agrobacterium-mediated transformation Endosperm–specific Fluorescence quantitative analysis
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 8
Quote: 0
Read: Download Dissertation

Abstract


Wheat is one of the world’s most important food crops, and its production is about 6 million tons every year worldwide,and provides daily requirements of protein and calories for more than 35% of the global population. Wheat processing quality is closely related to the composition of storage protein.Therefore,in order to improve its quanlity, transforming high-quality storage protein genes into wheat by genetic engineering methods is currently the main topic of wheat genetics and breeding. Isolation and characterization of endosperm-specific promoter which can drive high-quality wheat storage protein genes with high level of expression, will guarantee the realization of this goal. In the present research,by using the previously constructed five avenin-like b gene promoters to drive gus gene expression vectors with different deletions,the expression analysis of gus gene driven by these promoters was performed in transgenic tobacco.The main results are as follows:(1)Expression vectors of pALP-3, pALP-6, pALP-8, pALP-10, pALP-17 constructed by deletions with different lengths were transformed into tobacco by Agrobacterium- mediated transformation technology.And the positive transgenic plants were selected using PCR screening.(2)The results of GUS histochemical staining of positive transgenic plants confirmed that the avenin-like b promoter is endosperm-specific,and that the GCN4 and the Skn-Ⅰmotif are essectial for endosperm-specific expression.(3)Fluorescence quantitative analysis of transgenic positive plants showed that relative expression activities were 273.06 nmol/mg protein/hr, 314.50 nmol/mg protein/hr, 447.86 nmol/mg protein/hr, 233.74 nmol/mg protein/hr and 173.68 nmol/mg protein/hr, respectively for vectors pALP-3, pALP-6, pALP-8, pALP-10 and pALP-17. The transgenic tobacco plants which harbored 800 bp sequence of the promoter had the highest GUS activity.Isolation and functional analysis of wheat endosperm-specific promoters can contribute to study the mechanism of seed development and the regulation mechanism of endosperm-specific genes.The endosperm-specific promoters can be used to gene modification in plant genetic engineering.Thus, it not only has potential applications in theorey but also in practial applications.

Related Dissertations

  1. Screen and Analysis of Specific Expression Gene Promoter in Stem and Leaf of Rice,S511
  2. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  3. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  4. Polymorphisms in the Promoter Region of Swine BMP7 Gene and Their Association with Reproductive Traits,S828
  5. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  6. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  7. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  8. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  9. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  10. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  11. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  12. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  13. Cloning of Glyceraldehyde-3-phosphate Dehydrogenase Gene and Establishment of Agrobacterium-Mediated Transformation System of Rhizoctonia Solani,S435.111.42
  14. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  15. Resistance Analysis of the Code Region of Pib Gene to Blast in Transgenic Rice under Different Promoters,S435.111.41
  16. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  17. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  18. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  19. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  20. Effects of Pre-Anthesis Drought Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Drought and Its Physiological Mechanism,S512.1
  21. Difference in Development between Culm and Tiller Roots and Their Contribution to Grain Yield and Quality in Wheat (Triticum Aestivum L.),S512.1

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile