Dissertation > Excellent graduate degree dissertation topics show

The Isolation and Genetic Transformation of TaCBL3 in Wheat (Triticum Aestium L.)

Author: XuLang
Tutor: HeGuangYuan
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: Wheat TaCBL3 over-expression vector particle bombardment genetic transformation
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 11
Quote: 0
Read: Download Dissertation

Abstract


CBL proteins (calcineurin B-like protein) were identified as a group of calcium sensor unique to plants and some protozoa in recent years. CBLs activate the downstream calcium signaling transduction pathways through interacting with CIPKs ( CBL Interacting Protein Kinase). The Ca2+ -CBL-CIPK signaling pathway plays important roles in response to various abiotic stresses such as high salt, drought, low temperature in planta.In the present research, a full-length cDNA sequence encoding TaCBL3 was cloned. TaCBL3 over expression vector was constructed and delivered into developing wheat scutulum by particle bombardment.The main results are as follows:(1) We first searched the ESTs of TaCBL genes in database and then assembled them into TaCBL genes. Specific primers were designed and a TaCBL cDNA sequence was cloned by RT-PCR.(2) Sequence alignment and phylogenetic tree analysis showed that this gene was highly homologous to OsCBL3, therefore it was designated as TaCBL3 gene. Arabidopsis and rice CBL proteins generally have four EF-hand structures, while only three EF-hand structures were found in TaCBL3.(3) It is reported that the OsCBL3 gene plays a important role in multiple abiotic stresses, such as salt, drought and cold. Therefore, in order to further study the role of TaCBL3 in abiotic stress, and to breed new wheat varieties which are resistant to abiotic stresses, TaCBL3 gene over-expression vector was constructed by inserting it into pAHC25, and the over-expression vector was introduced into developing wheat scutulum in Zhengmai 9023 by particle bombardment, and regenerated transgenic wheat was obtained.(4) The vector of pAHC25 contains the bar gene, so we identified the transgenic plants of wheat through the PCR amplification of bar gene, and gained seven positive transgenic wheat.The results thus obtained in this research laid the base for further investigating the role of TaCBL3 in response to abiotic stresses in wheat.

Related Dissertations

  1. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  2. Improvement of the Resistance of Nicotiana Tabaccum to Virus by RNAi and Initial Establishment of Genetic Transformation System of L.regale,S435.72
  3. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  4. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  5. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  6. Establishment of Inderect ELISA for Detection of Antibodies Angainst STLV-1 and Preparation of Hybridoma Cell of Monoclonal Antibodies,R373
  7. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  8. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  9. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  10. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  11. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  12. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  13. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  14. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  15. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  16. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  17. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  18. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  19. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  20. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  21. Effects of Pre-Anthesis Drought Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Drought and Its Physiological Mechanism,S512.1

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile