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Construction of RNA Interference Vectors of Pin Genes Determining Grain Hardness and Genetic Transformation in Wheat

Author: ZouLiangYing
Tutor: YangGuangXiao；HeGuangYuan
School: Huazhong University of Science and Technology
Course: Biochemistry and Molecular Biology
Keywords: Wheat Grain hardness PinA PinB RNA interference Genetic transformation
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


Wheat Hardness is one of the most important traits in wheat processing quality is determined , it has a great impact on wheat flour yield , starch damage and water absorption of the raw dough . Wheat Hardness is mainly located on chromosome 5D short arm of two genes Puroindoline of a , b (PinA and the PinB) control , PinA , and PinB gene mutations or deletions can cause increase in the hardness of wheat endosperm texture . Pin gene function studies and transgenic applications on wheat quality improvement theory and practice are important. In this paper, by RNA interference technology , so PinA , and PinB gene transcriptional silencing occurs , Puroindoline protein expression was decreased to reach ordinary hexaploid wheat grain hardens purpose . This will further study wheat PinA , the foundation PinB affect Grain hardness and biological function ; hexaploid durum wheat this new type of germplasm for breeding high-quality wheat for different end uses provides a good germplasm resources . Constructed in this study the specific cause PinB gene silencing RNAi vectors , combined with the laboratory prior periods to build good PinA gene RNAi vector hexaploid wheat L88-31 receptor material , three different plasmids the combination genetic transformation experiments : RNAi vector PinA , PinB of RNAi , carrier PinA of RNAi vectors PinB RNAi vectors ; gene gun mediated guide the genetic transformation method , get transferred gene regenerated plants ; utilize PCR technology to detect T0 generation of transgenic plants exogenous fragment , obtained positive transgenic plants . The results of this thesis ultimately hexaploid new varieties of durum wheat , as well as clarify PinA PinB laid the foundation for influence grain hardness and biological function .

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CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
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