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Objective: The virus serum HBV mutant and wild-type strains infected HepG2 and L02 cells to evaluate whether these two cells can be HBV infection , and to explore whether there are differences in the ability to both copy . Methods: collect ADV mutants of LAM mutants and the wild-type strain of HBV infection in patients with serum . Incubated with 2% DMSO solution culture human hepatoma cell line HepG2 cells and normal liver cell line L02 cells four days later , both cells were infected with serum collected after infection 0,1,2,4,6,8 10 days to collect cell supernatants using time-resolved fluorescence immunoassay to detect cell supernatant HBsAg levels ; quantitative real-time PCR method to detect cell supernatant HBV DNA levels . Collected after infection 0,24 h , 48h , 96h time point , cells , immunofluorescence assay cells HBcAg positioning . Results : 1 of HepG2 and L02 cell supernatant HBsAg content showed an increasing trend , peaked at 2 days after infection , and then decreased gradually . Amount of HBV-DNA in the two cell supernatant showed an increasing trend , peaked in the first four days after infection , the downward trend in the future ; 3. 24h to see a strong signal for the first time in two cytoplasmic HBcAg, evenly distributed , while no positive expression in the nucleus ; 48h can see the red marker in the nucleus and cytoplasm HBcAg expression weakened ; nuclei and cytoplasm of HBcAg expression decreased after 96 hours . ADV mutants , LAM variant strain , HBV wild strains of virus in serum was detected after infection of HepG2 cell supernatant HBsAg and HBV DNA levels , respectively , the difference was statistically significant ( p lt; 0.05 ) . ADV mutant LAM mutants compared to results without statistical significance ( p gt ; 0.05) , compared to the other two in two results were statistically significant ( p lt; 0.05 ) . ADV mutant LAM mutant HBV wild strains of virus serum infection L02 detection of HBsAg in cell supernatants amount compared to each other , the result was statistically significant ( p lt; 0.05 ) . Which ADV mutants compared to wild-type strain of HBV , the results were statistically significant ( p lt; 0.05 ) , compared to the other two in two showed no statistical significance ( p gt ; 0.05) . Conclusion : virus serum HBV mutants and the wild-type strain can infect HepG2 and L02 cells , the replication capacity of HBV mutants weaker than HBV wild-type strain .
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