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Shigella (Shigella spp.) The foodborne bacteria caused by invasion of the intestinal epithelial cells of patients with typical of dysentery symptoms (fever, abdominal pain, diarrhea). Sonnei Shigella (S. sonnei) is one of the main pathogens of dysentery popular. Sonnei Shigella presents many differences due to the different environments of the various regions. This issue through four comes from a comparative study of the different shigella (S09071, S09072, S09076, S09077), provide some guidance on the the Shigella pathogenicity clinical disease control and epidemiological research. Deletion mutant library building can better study the virulence plasmid and chromosomal genome interaction between the virulence of Shigella plasmid, and also lay the foundation for the study of Shigella pathogenesis. Firstly, the four sonnei Shigella (S. sonnei) S09071, S09072, S09076, S09077 from morphology, serum agglutination and virulence evaluation, growth characteristics, physiological and biochemical characteristics, six resistance do a detailed Comparative Study; followed by detection of virulence genes, two-dimensional electrophoresis, whole cell protein expression profiles compared from the point of view of the molecule further comparative study of four strains; Finally, do differences in protein expression profiling protein MALDI-TOF/TOF identified by mass spectrometry. On the other hand, the paper has also established a large fragment of the Shigella virulence plasmid knockout. Through these studies, made the following main results: (1) the four Shigella sonnei form was straight rod-shaped spores, capsule, flagella and fimbriae, bacterial surface morphology uneven size of 0.5 to 0.7 × 1 ~ 2μm. Colonies in LB medium was a large round, flat, smooth, moist, colorless, translucent edges neat size of about 2.5 mm. Four of bacteria bacterial and colony morphology is almost no difference. (2) the growth curve is the fastest growing S09071, S09076, S09077 Secondly, S09072 strain growth slowest. (3) slightly different biochemical properties, S09076, S09077 can rhamnose, L-rock sugar, Arbutin, been Qiyeling fermentation S09071, S09072 can not use. (4) resistance found S09071, S09072 than S09076, S09077 two bacteria more resistant, which S09072 resistance strongest. (5) The virulence evaluation found that only S09072 epithelial cell invasion, S09071, S09076, S09077, no invasion force. (6) virulence genes detected S09071, S09076, S09077 part virulence gene is lost; only S09072 virulence gene complete. (7) by comparative proteomics research found that S09071, S09072 whole cell protein expression profiling, while S09076, S09077 whole cell protein expression profiles are consistent. S09071, S09072, S09076, S09077 between 48 distinct protein differences between 47 scores protein spots identified by mass spectrometry, corresponding to 43 proteins,, such as GadA GadB of OmpA, TatB such as these proteins in bacteria play an important role in the process of metabolism, virulence play and tolerance to different environmental. (8) successfully established a the knockout method Shigella virulence plasmid large fragment, laid the foundation for the pathogenic mechanism of Shigella virulence plasmid function Shigella.
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