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Study on the Characters of Hemoglobin by Recombining Hemoglobin Using Chemically Modified Heme

Author: LingHuiSheng
Tutor: LiRenQiang
School: Jinan University
Course: Biochemistry and Molecular Biology
Keywords: Chemically modified Heme Hemoglobin Peroxidase Enzyme activity
CLC: Q51
Type: Master's thesis
Year: 2011
Downloads: 47
Quote: 0
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Abstract


Peroxidase peroxide scavenger cells , cell protection , anti-oxidation, anti-aging effect , there is a wide range of applications in the food and cosmetic additives and biochemical reagents and other fields . But the high temperature of the natural enzyme , the strong acid and alkali - resistant performance difference , mutability inactivation , the harsh reaction conditions , the catalytic efficiency is not high in an organic solvent , in the practical application price is very expensive , thus limiting the peroxidase in the industrial on the application . On the basis of access to large amounts of relevant literature , the authors determine the structure and properties of the more thorough study a wide range of sources hemoglobin changes on the molecular level , the cofactor modification of hemoglobin . The use of phenol or chemical modification of hemoglobin heme hydroxybiphenyl modified heme and apo- hemoglobin hemoglobin reassembly . The light absorption scan analysis of the modified heme and reassembly hemoglobin proved a new reassembly hemoglobin building success . Enzyme assays , the modified heme reassembly hemoglobin class peroxide enzyme activity than natural hemoglobin high enzyme activity , with heme reassembly hydroxybiphenyl modified hemoglobin enzyme activity improved significantly , natural the hemoglobin is about 1.6 times . Found that the effects of temperature on hemoglobin and different phase high temperature such as 4-Biphenylol Hb in 40 ° C water bath , water bath 10min after sampling, enzyme activity was measured and found to reassemble reassembly of a chemically modified hemoglobin the enzyme activity of the hemoglobin can be maintained at a level of about 80% to about 20% higher than the activity of natural hemoglobin , which illustrates the reassembly hemoglobin heat performance is improved . Research samples tolerance when in the five kinds of the organic solvent of the acetone, acetonitrile, dimethylsulfoxide , ethanol and tetrahydrofuran, etc. , found reassembly hemoglobin tolerance stronger than the natural and controlled hemoglobin , relative activity of the residue to be high , and with the concentration of organic solvent and the enzymatic activity caused by the rate of decline is relatively slow, the rate curve falls relatively flat .

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