|
Purpose The purpose of this project is to study the effect of Pu'er tea joint calorie restriction tumor rat model of breast cancer prevention, suppression or treatment of cancer, and extend its lifetime, and their joint effect of applied common teas comparison with the other two, may explore Pu'er tea anti-cancer mechanisms and try and watch its tumor suppressor is an effective way to reduce heat this way, Pu'er tea and caloric restriction for the further development of the use of tumor suppressor provide a theoretical basis for data. Experimental methods, calorie restriction best suppressor effect on breast and colorectal cancer, Pu'er tea inhibitory effects on breast cancer cells is particularly evident: Pu'er tea with different concentrations of dissolved media processing murine colon cancer cells and to do MTT assay, depending on whether there is inhibition of cancer activity, if you can build a rat model of colorectal cancer, animal experiments. 2, Pu'er tea joint caloric restriction treatment of experimental studies of animal tumor suppressor effect: making good rat model of breast cancer, Pu'er tea and water, and were given free access to food group and caloric restriction group divided into weekly weighing The body weight of rats, measuring changes in tumor volume in rats records rats survival of weighing in rat liver after treatment, kidney weight, spleen weight, etc., of serum biochemical indicators in rat liver and tumor tissue do He biopsy, RT-PCR and immuno histochemical analysis of a key enzyme in glucose and lipid metabolism, comprehensive study evaluating its tumor suppressor role and explore its possible tumor suppressor pathway. 3, animal prevention of experimental of Pu'er tea joint calorie restriction tumor suppressor effect: in a rat model of breast cancer before giving the tea, and the purposes of calorie intake limit a month, after modeling continue to give this two month detect project ibid., comprehensive studies on the prevention of cancer and the tumor suppressor role and explore its possible tumor suppressor pathway. 4, green tea, black tea, tea oxidation resistance comparison: ORAC value of detection 5 tumor suppressor effect of green tea, black tea, tea joint calorie restriction comparative study (ibid., 2 and 3, including treatment experimental and prevention experiments) around: a rat model of breast cancer, began to give the same concentration of green tea, black tea, tea treatment, combined with caloric intake limit this impact factors, weight of rats by weekly weighing, measuring the rat tumor volume change record rats survival period after the end of treatment, weighing liver weight, kidney weight, spleen weight, etc., of serum biochemical indicators HE biopsy on liver and tumor tissue, RT-PCR and immunohistochemistry. analysis of a key enzyme in glucose and lipid metabolism, the comprehensive evaluation Zhongcha tumor suppressor role and explore its potential tumor suppressor pathway. The experimental results, four concentrations of Pu'er tea of ??murine colorectal CT-26 cells did not inhibit growth. 2 a day, 30% of calorie intake to a certain extent, prevent, inhibit tumor, slowed tumor growth and prolong the survival time of tumor in rats fed 2.0g/kg body weight of Pu'er tea joint restrictions. The prevention group tumor suppressor effect is better than the treatment group. 3, tea (2.0g/kg) joint calorie restriction (30%) can significantly rats play a lower body weight, fat, lowering blood sugar, the biopsy can promote apoptosis of tumor cells may affect glucose and lipid metabolism key enzyme acetyl coenzyme A carboxylase (ACC), fatty acid synthesis enzymes (FAS), plus at the same time the reduction of caloric intake, promote tumor growth insufficient supply of nutrients required to play a tumor suppressor role , and the combined effect than individual effect, no effect on the blood biochemical indicators joint application of normal rats. 4, three tea antioxidant activity in comparison: green tea gt; the Pu'er tea gt; tea. Prevention and suppression of tumor growth comparison: Pu'er tea gt; the green tea gt; tea. Experimental results, tea joint calorie restriction can play better against tumor effect, inhibiting tumor growth, prolong the survival time of rats, a significant improvement in the survival of the state of the tumors in rats. 2 Overall, the effect of the prevention group was significantly better than the treatment group. Visible long-term consumption of tea, but the degree of very good eating on the prevention of tumor and prolong life. 3, Pu'er tea tumor suppressor mechanism may be related to glucose metabolism and its key enzyme in the pathway protein levels and RNA levels significantly inhibited joint calorie restriction, suppression better. 4, green tea, black tea, Pu'er tea has certain prevent cancer and inhibit tumor efficacy, while green tea oxidation resistance optimal, but the terms of, the tumor suppressor effect of Pu'er tea is best, followed by green tea may inhibition of glucose metabolism pathway and Pu'er tea.
|