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Objective: To investigate cyclophosphamide and busulfan dose and mode of administration, seeking a better way of making a rat the NOA model of the rat spermatogenic function. Methods: 160 SPF level adult male Wistar rats were randomly divided into 10 groups, each group of 16, 1-3 respectively, be the busulfan 20mg.kg 15mg.kg-1 10mg.kg-1 single intraperitoneal injection of 4-6 were be the on cyclophosphamide 200mg.kg-1 100mg.kg 1, 75mg.kg-1 single intraperitoneal injection of 7-9 were busulfan 40mg.kg-1 , 20mg.kg-1, cyclophosphamide 200mg.kg-1 single ig group of 10 single intraperitoneal injection of to be saline 2.Oml line. The NOA model standard medication to rat reproductive cycle is about 38 days, rat testis, epididymis tissue sections sperm and sperm cells shall not find NOA model. Comparative model of success and saline groups, the body weight of rats model failure group and the saline group, testicle, epididymis weight and the number of spermatogenic cells significant difference, combined with the mortality of the rats, draw practical a model. Results: After 38 days of the first groups of rats survived for two, the second group of eight survived, the survival of three groups of 15, the survival of the four groups of three, five groups of 10 survived, the survival of the six groups of 15, survived a Group 7, Group 8 survived two Group 9 survived two saline rats no death. 38 days after treatment, the survival of rat testis and epididymis tissue slices results suggest 1,2,7,8 group to achieve the the NOA model's standard the remaining rats the NOA model of the effect of the poor. By way of comparison of the various components mold, the integrated model is successful and the lowest mortality by 15mg.kg-1 single intraperitoneal injection of busulfan practical Wistar rat the NOA model making way. Conclusion: 15mg.kg-1 single intraperitoneal injection of busulfan can successfully fabricated the Wistar rats NOA model and lower mortality. Objective: To assess FSH, SCF Wistar rats the NOA animal model spermatogenic function. Methods: busulfan 15mg. Kg-1 single intraperitoneal injection of produced 72 Wistar rats the NOA animal model, sampling check model results successfully. Model will be divided into two groups: the treatment group rat systemic blood FSH, SCF total of five times in the hind legs intramuscular injection in rats; the control group were given the same amount of rat hind leg intramuscular injection of normal saline; administered two one week times. Batches take 19 days, 38 days, 57 days after administration of rat testicle, epididymis line of tissue sections, testicle, epididymis made into a slice. Calculation of the seminiferous tubules vertical section of spermatogonia, spermatocytes, the number of sperm cells and sperm and vertical sections of the epididymal tube sperm count, each section observed compliant 25 seminiferous tubes. t test was used to compare the treatment group and the control group, the results of the different medication time to require P lt; 0.01 difference was statistically significant. Results: ① treatment group and the control group medication 19 days after an average of each of the seminiferous tubules spermatogonia number were 58.00 ± 15.70 and 30.90 ± 13.52, P = 0.000; the spermatocytes were 47.44 ± 15.52 and 12.65 ± 8.08, P = 0.000; sperm cells and sperm counts were 12.42 ± 8.11 and 0, P = 0.005. Experimental group and a control group of medication 19 days after the average sperm count in each epididymal tube, respectively, 0 and 0, P = 0. ② treatment group and the control group medication for 38 days after the average number of each seminiferous tubules spermatogonia were 66.77 ± 20.56 and 40.65 ± 15.89, P = 0.000; spermatocytes were 56.82 ± 21.93 and 18.50 ± 13.66, P = 0.000; sperm cells and sperm counts were 61.17 ± 25.86 and 27.65 ± 9.62, P = 0.002. Medication after 38 days the average sperm count in each of the epididymal duct of the experimental and control groups were 77.55 ± 55.18 and 41.05 ± 29.20, P = 0.000. ③ treatment group and the control group medication for 57 days after the average of each seminiferous tubules of spermatogonia were 78.63 ± 24.28 and 47.95 ± 18.12, P = 0.000; spermatocytes were 68.53 ± 21.54 and 29.60 ± 15.13, P = 0.000; sperm cells and sperm counts were 102.83 ± 35.31 and 45.15 ± 20.96, P = 0.000. Medication of the treatment group and the control group 19 days average sperm counts within each of the epididymal duct were 122.13 ± 80.67 and 58.50 ± 26.15, P = 0.000. Conclusion: intramuscular injection of FSH and SCF to promote the recovery of the Wistar rat the NOA model of spermatogenic function.
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