Dissertation > Excellent graduate degree dissertation topics show

Effect of BMP-2 Transfection Mediated by Adenovirus on the Bone Marrow Mesenchymal Stem Cells

Author: DingLin
Tutor: SunHongChen
School: Jilin University
Course: Stomatology
Keywords: Bone marrow mesenchymal stem cells Osteoblasts Differentiation Transgenic BMP2
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


Mandibular defects caused by tumors, trauma , inflammation , and other factors and absorption is a common clinical problem in the field of oral medicine . Osteoblast cells are the principal cells involved in bone formation , and their precursor cells in bone marrow mesenchymal stem cells under appropriate conditions can differentiate into a variety of cells such as chondrocytes , osteoblasts , adipocytes , and myoblasts . Regulation of differentiation and function of bone marrow mesenchymal stem cells into osteoblasts , has important theoretical significance not only for the correct understanding of the mechanism of bone formation , and further treatment of bone resorption diseases , promote bone regeneration has practical significance . Bone formation is affected by a variety of cytokines and growth factor regulation of the process and how to better promote healing of bone tissue growth factor , current research is one of the hot spots . Studies have shown that bone marrow mesenchymal stem cells and bone -derived growth factor can significantly promote bone regeneration . BMP-2 is formed as a bone regulatory factors , a significant role in the process of promoting fracture healing and bone defect repair . This study was to by transgenic methods to import exogenous BMP-2 bone marrow mesenchymal stem cells , to make it efficient, stable expression , in order to achieve the purpose of promoting bone formation . This study through the establishment of mesenchymal stem cells in vitro bone marrow culture method , constructed an adenovirus vector carrying BMP-2 gene transfection in vitro culture of bone marrow -derived mesenchymal stem cells . MTT, alkaline phosphatase activity assay and alizarin red staining was used to detect BMP-2 bone marrow mesenchymal stem cell proliferation and osteogenic differentiation , the results showed that BMP-2 can promote the proliferation of bone marrow mesenchymal stem cells , and can promote their differentiation into osteoblasts . Therefore , the establishment of this culture system of bone marrow mesenchymal stem cells vitro studies of bone marrow mesenchymal stem cells into the regulation of the differentiation and function of bone cells and to provide a realistic and practical approach ; study bone marrow mesenchymal stem cells transfected gene of feasibility , in order to further the use of transgenic technology to the treatment of bone resorption diseases , promote bone formation lay the theoretical foundation .

Related Dissertations

  1. Expression of D-AtCGS in E. Coli and Preparation of Polyclonal Antibody Against D-AtCGS,Q943.2
  2. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  3. Professor Wang Qi identified the body - of Diseases - dialectical combination of academic thought and clinical experience and treatment of chronic insomnia clinical studies,R249.2
  4. Research on Feed Safety of Transgenic Rice in Broiler,S831.5
  5. Soil Eoclogical Risk Evaluation of Transgenic Bt Rice Through Nematode Community Analysis,S154.1
  6. The Effects of Overexpression of OsSsr1 on Stress Tolerance in Tobacco,S572
  7. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  8. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  9. Study on Detection of Transgenic Ingredients in Genetically Modified Cottonseed Meal and Its Degradation during Feed Processing,S816
  10. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  11. miR - 23 in mouse skeletal muscle expression and the regulation of skeletal muscle regeneration research,Q78
  12. Evaluation of Land Use Eco-security in Fujian Province,X826
  13. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  14. Studies on the Differences of Tolerance between Chilo Supperssalis Walker and Sesamia Inferens Walker to Bt Rice,S435.112.1
  15. Genetic Characterization and Differentiation of Natural Populations of Wild Soybean from Middle and Lower Valleys of Huanghe and Changjiang as Well as Their Genetic Relationship with the Cultivated Soybean,S565.1
  16. Resistance Mechanism of Major Commercial Rice Varieties to RSV and Construction of Trangenic Rice to Fight Against Virus,S511
  17. Construction of the Expression Vector and Transformation of Ta-UGT3 Gene into Common Wheat,S512.1
  18. Studies on Photosynthetic and Physiological Characters of Transgenic Wheat with Maize High Photosynthetic Efficiency C4 Type PEPC Gene,S512.1
  19. Preliminary Function Analysis of Two Annexins in Cotton (Gossypium Hirsutum L.),S562
  20. Study on the Transgenic Tobacco Expressing Protein Kinase Like Gene TA50-10 of Wheat and Analysis of Disease Resistance,S572
  21. Resistance of hrfl Transgenic Wheat to Fusarium Graminearum and Construction of Rice RNA Silencing Vector,S512

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile