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Human herpes virus type 7 (HHV-7), and belongs to the family of β-herpes virus (DNA virus). The virus has characteristics of latent infection, epidemiological investigations around the world, in different parts of the crowd in the blood and saliva positive rate of 17% to 96%, suggesting that HHV-7 is a ubiquitous virus. HHV-7 infection and child care acute rash, central nervous system infections, organ transplantation, chronic EB virus-like infection, fatigue syndrome and acquired immune deficiency syndrome patients with co-infection, and lichen planus relevant. Primary infection subitum after primary infection, the virus can be in the body long-term potential, and organ transplant patients with HHV-7 reactivation may occur. The study aimed to establish real-time quantitative PCR (RT-PCR) to detect human herpesvirus 7 (HHV-7) and to evaluate the sensitivity and specificity of quantitative measurement of clinical specimens, allogeneic hematopoietic stem cell transplantation (( allo-HSCT) infection. 1 real-time PCR to detect human herpesvirus 7 method to establish the purpose of the establishment of a real-time quantitative PCR detection of human herpesvirus 7 for clinical HHV-7 gene quantitative detection method based on HHV-7 gene sequence primers and probes, and HHV-7 specific gene fragment was amplified, cloned recombinant pGM-T vector, a gene sequence, recombinant plasmid as a standard sample, a standard curve, the sensitivity and specificity of the detection Results PCR amplified a 128 bp fragment, sequencing the recombinant plasmid containing specific for HHV-7 gene sequence, the standard curve correlation coefficient of 0.9989, and real-time quantitative PCR amplification efficiency was 95.86%, and the detection sensitivity was 20 copies / reaction system. Conclusion successfully established HHV-7 gene real-time quantitative PCR method has high sensitivity and specificity, and can be used for the quantitative detection of clinical and research specimens HHV-7 gene. 2 allogeneic hematopoietic stem cell transplantation of human herpes virus type 7 infection Objective To explore the HHV-7 in allogeneic hematopoietic stem cell transplantation in patients infected with the status quo. collected 40 cases before allo-HSCT transplant and post-transplant patients with peripheral blood samples of 384 and 40 copies of donor specimens using real-time quantitative PCR donor pre-transplant and post-transplant patients with human herpes virus type 7 40 allo-HSCT donor DNA positive rate was 32.5% (13/40), the median viral load for 271EqCop/106PBC (37 ~~ 1095); 40 allo-HSCT recipients, pretransplant DNA positive rate was 37.5% (15/40), the median viral load 285EqCop/106PBC (47 to 3764); 40 allo-HSCT recipients, 344 specimens, transplant after DNA specimens positive rate of 43.90% (151/344), the median viral load for 457EqCop/106PBC (41 to 5218), significantly higher than the donor virus load (p lt; 0.05) HHV-7 DNA positive rate gender, age, and the basis of diseases such as non-significant with correlation. recipients after transplantation of HHV-7 infection and for those who, by the by pre-transplant infections significantly with related (p lt; 0.05). conclusion HHV-7 infection in patients after allo-HSCT very common in their viral load is higher than the donor. HHV-7-positive donor and recipient before transplantation recipients after transplantation, HHV-7 is more prone to infection, and therefore donor, transplant recipients before HHV-7 positive after transplant patients require detection of HHV-7.
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