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Comes from the dried ripe seeds in seeds, Amaranthaceae species in seeds (Celosia argentea L.) in seeds, only contained in the \Bitter, slightly cold, pass through liver. Liver, eyesight back shade of efficacy. For liver heat, red eyes, look unfamiliar shade film, visual dim, anger vertigo. Early in the research group from the seeds of the four pentacyclic triterpenoid saponin compounds in seeds glycosides A green suitcase glycosides B, C in seeds glycosides and in seeds glycosides D, based on as continuity of work, continue to the Green study the chemical composition of the seeds of the first use of HSCCC-ELSD method fumble countercurrent chromatography solvent system partition coefficient and solvent selection software, the application of HSCCC accumulated some experience, and ultimately get the 10 compounds. Application of physical and chemical constants and spectroscopic methods (mp, IR, 1 H-NMR 13 C-NMR, HMBC, HMQC, COSY, NOESY, EI-MS, HR -ESI-MS) identified the chemical structure of the compounds 8, the five compound is a new compound, respectively named in seeds glycosides C 1 (the Celosin C 1 ) in seeds glycosides D 1 (Celosin D1), the green suitcase glycosides E (CelosinE), the the green suitcase glycosides F (Celosin F) and in seeds glycosides G (Celosin G); 1 for the first time to share , for the cockscomb glycosides (Cristatain). In seeds glycosides A, green suitcase glucosides B, the green suitcase glycosides C 1 the the green suitcase glycosides D in seeds glycosides E green suitcase glucoside F, in seeds glycosides 8 G and cockscomb glycosides compounds in vitro antitumor activity study results show that, in seeds glycosides A the green suitcase glycoside B, and cockscomb glycosides of five tumor cell SHG44 (human glioma cells), HCT116 (human intestinal cancer), CEM (human leukemia cell) of MDA-the MB-435 (human breast cancer cell), and HepG2 (human hepatoma cells) IC 50 value: SHG44 (35.37 ± 1.36 × 10 -6 mol / L, 21.65 ± 1.16 × 10 -6 mol / L, 35.82 ± 2.96 × 10 -6 mol / L), HCT116 ( 38.69 ± 1.86 × 10 -6 mol / L, 36.31 ± 3.69 × 10 -6 mol / L, 40.36 ± 4.11 × 10 -6 mol / L), CEM (39.82 ± 2.74 × 10 -6 mol / L, 24.43 ± 1.46 × 10 -6 mol / L, 47.76 ± 2.66 × 10 -6 mol / L), MDA-MB-435 (38.72 ± 3.24 × 10 -6 mol / L, 12.68 ± 1.08 × 10 -6 mol / L, 41.74 ± 2.93 × 10 -6 mol / L), HepG2 (39.53 ± 2.94 × 10 -6 mol / L, 31.86 ± 3.55 × 10 -6 mol / L, 42.82 ± 2.32 × 10 -6 mol / L), show that the three compounds have good anti-tumor activity, and in seeds glycosides C1, Green and Celosia argentea glycosides D1 in seeds glycosides E, green suitcase glucoside F, green suitcase glucoside G no significant anti-tumor activity of the five kinds of tumor cells. Carbon tetrachloride-induced liver injury model in seeds glycosides C 1 and green suitcase glycosides D 1 vivo hepatoprotective activity. The results showed that the the blue suitcase glycosides C and in seeds glycosides D 1 7 days continuous oral administration of CCl 4 cause of AST, ALT liter high significant preventive effect, significantly improve the carbon tetrachloride liver damage caused by liver tissue SOD, CAT, GSH level of vitality and reduces lipid peroxidation product MDA content in 2.0mg kg - 1 -8.0mg · kg -1 range with the control group, there are very significant differences (P lt; 0.01). Biopsy revealed model mice liver tissue showed focal necrosis, necrosis of liver cells structure has been severely damaged, the basic outline of the liver cells disappear, with massive lymphocyte predominant inflammatory cell infiltration in high doses clear lobule structure of the group of animals, arranged in a liver cell disorders, degeneration of cells of the intrahepatic occasionally watery, almost no inflammatory cell infiltration. The green suitcase glycosides C and oral in seeds glycosides D 1 CCl 4 biochemical changes caused a significant preventive effect. The results of this study to lay the foundation for the rational development and utilization of green seeds.
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