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The Complexation and Dissociation Mechanism Study of Pb Serumn Protein Complex Based on Electrochemical Method

Author: LiangYing
Tutor: DongTieYou
School: Henan University of Science and Technology
Course: Chemical processes
Keywords: Lead Bovine serum albumin Complex Simulate blood Dialysis Electrolysis
CLC: R114
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
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Abstract

In recent years, more and more reports of lead contamination and lead to harm to human (especially children) at home and abroad. Lead is a persistent pollutants in the natural environment can not be decomposed by biological metabolism. Lead for most systems of the human body are hazards, especially bone marrow hematopoietic system, nervous system and kidney injury. Lead into the human body, in addition to the part in the faeces, sweat excretion, and the remaining hours later dissolved in the blood, impede blood synthesis. The serum protein is a major component of the plasma protein, having a storage and carrier functions. The prevalence of serum proteins in the blood is purified and easy, so it's become a scientist one of the earliest studies of protein. Lead and serum protein from the molecular level to explore the mechanism of action and research lead method is the this topic starting point. First, this study of the role of the lead with bovine serum albumin (BSA) in neutral conditions mixed system fluorescence spectroscopy characterization, the following conclusions: Pb ^{2 static quenching quenching of BSA Off-based, i.e. in the buffer solution system Pb generated with BSA complexes. Secondly, the present study using conventional dialysis to reduce the concentration of lead in the neutral condition Pb-BSA complex. The test is made of lead nitrate solution and the BSA mixture as an analog blood poisoning, and then using the laboratory production of simulated dialysis apparatus to reduce the concentration of lead poisoning simulated blood to detect simulated changes in the concentration of lead in blood and the dialysis process. The test results show that: under acidic conditions of the poisoning of simulated blood through dialysis, wherein the lead concentration decreased rapidly, the final concentration of lead is reduced to a very low value; under a neutral condition, the poisoning of simulated blood through the dialysis, the concentration of lead decreased slowly, and finally lead concentration is maintained higher stable value. The test results show that it is not feasible to reduce the concentration of lead in the Pb-BSA complex system with conventional dialysis. Again, the design of the dialysis apparatus of this study using a laboratory, under neutral conditions poisoning analog blood dialysis, removal wherein the uncomplexed lead ion and other impurities small molecule design their own, and then using a laboratory electrolytic means to simulate blood poisoning electrolysis, and finally again with a dialysis apparatus, after electrolysis analog blood for dialysis, and the change in the concentration of lead in the entire process for testing. The test results show that the electrolysis can effectively reduce poisoning simulate blood lead concentration after electrolysis of lead is deposited on the electrode plate. Finally, the above analysis can be drawn: under physiological conditions lead nitrate to generate a large number of complex and BSA; the traditional dialysis method to reduce the concentration of lead in the Pb-BSA complex system does not work; electrochemical artificial kidney device can effectively reduce the concentration of lead in the analog blood poisoning, electrolysis method is an effective deleading.}

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