Dissertation > Excellent graduate degree dissertation topics show

Studies on the Cortical Ciliature and Pigment Granule of Blepharisma Americanum(Heterotrichida, Ciliphora)

Author: ZuoJuMin
Tutor: GuFuKang
School: East China Normal University
Course: Zoology
Keywords: Different hair mesh U.S. Zheshan fiber insect Cortical microtubular organelles Morphogenesis Flutax direct fluorescent labeling Cortical pigment granules
CLC: Q954
Type: Master's thesis
Year: 2011
Downloads: 7
Quote: 1
Read: Download Dissertation

Abstract

Protozoa ocher fiber Genus (Blepharisma) the First by the protozoan scientist of Switzerland Perry in 1852 established nearly 160 years has been the study of history. After a long research is now generally believed that the occurrence of more than one source system, Corliss different hair project is divided into six sub-orders: iso the gross suborder Heterotrichina, armed the suborder Amophorina, the test of Leah project Coliophorina mouth area cilia icon; Like Ya Head Licophorina; hypotenuse suborder Plagiotomina and six suborder attributed rotating lip subclass Spirotricha, Lynn the different polychaete only contains different hair mesh. Usually individuals of different hair mesh ciliates, visible, easy to cultivate, there is a certain degree of scalability, strong regenerative capacity, making them ideal study object of experimental ciliates across the surface of the same type of cilia. The current research is also focused on the injection organelles mucus bubble, bubble of barbed wire, and some other special organelles such as the symbionts, pigment granules uncommon. These functional organs have respective different structures and functions, the different schema similarity cilia function and structure are not identical. Collected in the field, the laboratory protozoa process acquired a different hair mesh ciliates ocher fiber insect, its morphological characteristics and Suzuki (1953) found the ocher-fiber insects (Blepharisma americanum), will be given for the species. Of ocher fiber insect study, research more ocher fiber insect pheromone composition and function, polymerase, Y-tubulin gene fragment and genetics research, and the cortical microtubules of ocher fiber insect cell morphology occurred and body cilia in cell structure formation process of differentiation characteristics is not fully clear. Direct fluorescent labeling method of this paper Flutax fluorescent paclitaxel ocher fiber morphology of insects cells the process of doing a detailed observation. The use of differential interference phase-contrast microscopy shows the parasite surface membrane epithelium pigment granules by the morphology and cell swelling to rupture the cell structure in strong light. At the same time the use of scanning electron microscopy technique Transmission electron microscopy ultrastructure observation of the United States ocher fiber insect cell surface and internal structure. American ocher fiber insect cortical microtubular organelles in the morphology and morphogenesis application FLUTAX the fluorescent paclitaxel direct fluorescent labeling, the United States ocher fiber insect cells were full of pear-shaped and presents a bright brick red slightly smaller, front, back-end obtuse. The peristomial with about 1/2 of the entire cell length cells, starting at the front end and extending to a cell in the central part, located in the cells in the left side of the ventral surface of the front end. An undulating membrane, located in the right side of the parasite cell length fluctuations film of approximately 2/3 the peristomial with length. Surface covered with the same type of cilia, the body cilia evenly distributed throughout the parasite cell surface and longitudinally arranged along the worm, the dynamic fibrils runs through the whole body is under the surface membrane. The process of cell morphogenesis, after Aberdeen insect population microtubules in the cortex mouth shroud first column body cilia position, the former Aberdeen insect population microtubule formation in the old port shrouds position; after Aberdeen insect population has occurred earlier than laokou Wai with updated old the peristomial with new peristomial Belt gradually updated. The body cilia cilia in various old columns occurred between the middle of every two cilia matrix, after which new the old body cilia composed the body cilia column of the new Aberdeen insects. The results the old the cell cortex cilia structure positioning and induced new cilia microtubules occur not only has the substance and structure of the contribution, and the formation of new cells. The former Aberdeen insects peristomial with experienced physiological reorganization cilia collapse of the update process, eventually forming the former Aberdeen insects new cilia. 2 ocher fiber insects cortical pigment granules microscopic observation using differential interference contrast microscopy, ocher fiber insect nutrition cells strong scalability, presents a bright red ocher, the cell surface membrane visible under darkfield The clear the telescopic bubble and the food bubble, rich red ocher fiber insect cells revealed subcortical pigment granules, they maintain the color of the cell. Cortical pigment granules of uniform size homogeneous spherical particles uniformly arranged in the cell surface membrane, and the filaments are arranged in pairs along the cell longitudinal axis of the body the cilia column next move. Dark field observation, the parasite cells swimming rapidly active subcortical pigment granules visible. When seen in a gradual strengthening of the bright field, the the cortical cells pigment particles due to the high intensity light irradiation, the pigment particles will rupture and accompanied by cell lysis, cytoplasmic outflow, cell rupture and death. According to the results suggest that: the United States ocher fiber insect pigment granules and cell color, pigment granules in the subcortical by the bright light of programmed cell death may be the cells of a self-defense style. 3 American ocher fiber insect cell ultrastructure observation. The technique of scanning electron microscopy, and transmission electron microscopy technique, ocher fiber insect cell surface spines spine between adjacent cilia ditch the body cilia Born cilia trench. Parasite cell surface is covered with the same type of long and intensive body cilia, and vertical uniform distribution on the body surface. Further magnification observation found that the smooth, normal state of the body surface area, no granular projections. Sometimes observed in the surface of the parasite cells to the small circular dimples. In conditions of poor nutritional status, body cells through the combination of the reproductive way to reproduce. The peristomial with by the cilia arranged in parallel columns in each other, and each cilium column by two parallel cilia rod. U.S. ocher fiber insect cells within the subcortical pigment granules and symbiotic bacteria structure is very rich, in addition to containing the structure of the nucleus, mitochondria, endoplasmic reticulum, the intracellular membrane system is not very developed, contains many oval food vacuole, and many did not digestion of food particles. The mitochondrial large number of very rich, a lot of particulate matter within the mitochondria. The cross-section shows cilia rod is also typical of 92 structures, subcortical pigment particle size uniformity evenly distributed in the surface membrane and cristae evenly arranged in accordance with each of the two group's way. Cytoplasm similar symbiotic bacteria teardrop-shaped substance not yet fully digested food bubble move fibrils arranged longitudinally along the parasite cell surface membrane. Peristomial with cilia arranged with some regularity, every two cilia rod composed of a double-action-based system, the majority of pigment granules in accordance with each of the two press distribution in the double-action between the base lines, sometimes visible three pigment particles are arranged in the mouth surrounded with cilia between.

Studies on the Cortical Ciliature and Pigment Granule of Blepharisma Americanum(Heterotrichida, Ciliphora)

Abstract

Related Dissertations