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Construction of ATSRRK1-4 and Preliminary Analysis of the Phenotype of ATSRRK5 in Arabidopsis Zuo-yuanyuan(Botany)

Author: ZuoYuanYuan
Tutor: LiXiaoPing
School: Huaibei Normal
Course: Botany
Keywords: Arabidopsis ATSRRKs Transgenic LRR-RLK Vector construction
CLC: Q943.2
Type: Master's thesis
Year: 2011
Downloads: 37
Quote: 0
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Abstract


Leucine-rich repeat receptor protein kinase (leucine rich-repeat receptor-like kinases, LRR-RLK, LRK) is the largest family of receptor-like protein kinase subfamily . Numerous studies show that class family of some genes in plant growth and development in the regulation of tissue formation and organ development , regulation pollen self -incompatibility , feel the plant pathogenic signals , as well as participate in intracellular signal transduction and stress response . LRRVIII-2 the LRK family subfamily , may also play an important role in the process of Arabidopsis Growth . The experiment by means of bioinformatics , respectively, of the coding sequence of the target gene and gene-specific sequence analysis of ATSRRK1-4 RNAi the carrier and ATSRRK1 and ATSRRK4 over expression vector was successfully constructed , and successfully obtain transgenic Arabidopsis plants . In PATSRRK1 : GUS transgenic Arabidopsis seedlings , GUS staining showed strong expression in the aerial parts of Arabidopsis underground part of the expression of the weak . 6-BA treatment PATSRRK1 : GUS transgenic Arabidopsis materials dyed than untreated group little change ; ABA treatment , compared with the untreated group dyed lighter . ATSRRK5 over-expression and RNAi transgenic material has been successfully obtained a homozygote . Chlorophyll content measurement results showed that the chlorophyll content in the dark : RNAi-ATSRRK5 , and ATnap5010c gt ; wild-type gt ; OV- ATSRRK5 and of arr1 , 2 . Measured by GUS staining and chlorophyll content results suggest LRRVIII-2 subfamily may be involved in plant growth and development and signal transduction . But specifically how to participate in the growth and development of plants and signal transduction pathways , needs further study prove .

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant Genetic Engineering
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