Dissertation > Excellent graduate degree dissertation topics show

Extraction and Purification of Mulberry Leaf Flavonoids and Its Hypouricemic Activity

Author: WangZuo
Tutor: ZuoWeiZuo
School: East China Normal University
Course: Botany
Keywords: Flavonoids Macroporous resin Adenine Xanthine oxidase Allopurin
CLC: TQ461
Type: Master's thesis
Year: 2011
Downloads: 143
Quote: 0
Read: Download Dissertation

Abstract


Leaves from Morus alba L.is a kind of traditional Chinese medicine. Mulberry leaf flavonoids are the mainly active ingredient in mulberry leaves. The technique of extraction and separation of active ingredient has been investigated in this paper. Thenceforth, the hypouricemic activity of the mulberry leaves flavonoids was explored.Mulberry leaf flavonoids were analysed with high performance liquid chromatography. Rrutin, myricetin, quercetin, kaempferol are the main ingredients in it. Factors of temperature, extraction time, ethanol concentration,volume of solvent and extraction frequency were evaluated for their effects on extraction process by orthogonal designed experiments. The results showed that the optimum conditions were 80℃,2 times,1.5 hours,70% ethanol-herb ratio of volume to mass was 20:1 respectively.In order to get purer mulberry leaf flavonoids, macroporous resin was adopted. The adsorption ability of macroporous resin was evaluated by yield and purity of the flavonoids. The kind of HPD826 was the best of the five macroporous resin. The optimum process conditions were 3 BV 60% ethanol as eluting solvent, adding sample rate in about 2 BV/h, eluting rate in about 1 BV/h. The yield of Mulberry leaf flavonoids by this process was 1.95%,and the purity was 55.41%.Pharmacology study showed that mulberry leaf flavonoids could adjust the activity of a-glucosidase, lower blood glucose, antitumor, etc. Results indicated that MLF inhibit rat liver XO activity on dose dependent. Normal mice were administered orally with MLF at doses of 50,100,150 mg./kg*d-1.The results showed that mulberry leaf flavonoids had no significant effect on uric acid levels and liver XO activity in normal mice.Experimental animal model of hyperuricemia was induced by uricase inhibitor potassium oxonate. Potassium oxonate-induced hyperuricemic mice were administered orally with MLF at doses of 50,100,150 mg/kg*d-1, while distilled water and allopurinol at 50 mg/kg*d-1 were given to the hyperuricemic control group and positive control group. The results showed that oral administration of 200 mg/kg*d-1 MLF to hyperuricemic mice exhibited significant reduction in the blood uric acid levels 16.67% and in XO activity 6.05%. The oral administration of 50 mg./kg*d-1 MLF also caused remarkable reduction in the blood uric acid levels, but without remarkable reduction in XO activity.Long-term hyperuicemia caused kidney damage and gout, renal damage cause uric acid excretion obstacles. We studied the hypouricemic effect of MLF by using model of adenine-reduced hyperuricemia in rats. In this test, Adenine-induced hyperuricemic mice were administered orally with MLF at doses of 50,100,150 mg/kg*d-1, while distilled water and allopurinol at 50 mg/kg*d-1 were given to the hyperuricemic control group and positive control group. The results showed that oral administration of 100 mg/kg*d-1 MLF to hyperuricemic mice exhibited significant reduction in the blood uric acid levels at different time periods:17.83% at 7th d, 21.17% at 14th d, and oral administration of 200 mg/kg*d-1 MLF caused remarkable reduction in the blood uric acid levels at different time periods:21.09% at 7th d, 27.15% at 14th d. Oral administration of 200 mg/kg*d-1 MLF also caused remarkable reduction in the blood triglyceride levels 41.95% and in the blood free fatty acid levels 42.05% at 21th d. Transmission electron microscopy (TEM) observation results showed that MLF protected kidney cells of rats which treated by adenine.All the results mentioned above showed that MLF could decrease the levels of blood uric acid. It also protect kidney cell and prevent it from further damage, improve the metabolism of triglyceride and adjust body weight in hyperuricemic mice. It was concluded that MLF had good activity on adenine-reduced hyperuricemic rats, but its molecular mechanism need further study.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
  3. Studies Preparation of Procyanidines from Grape Seeds and Mycotoxins Determination,TQ461
  4. The Study on Anti Tumor and Anti-oxidation Activities in Vitro of Extracts from Oxalis Corniculata L,R285
  5. Studies on Fingerprint Analysis and Total Flavone Extracts of Saussurea Involucrata ( Kar. Et Kir.) Sch.-Bip.,R284.1
  6. Initial Studies on the Antibacterical Activity in Vitro and the Safety Evaluation of Propolis Extraction,R285
  7. Study on the Preparation Technology and Quality Standard of Tongmai Drop Pills,TQ461
  8. To Study the Techniques for Purification of Nature Products by High-speed Counter-current Chromatography(Ⅱ),R284
  9. Effect of Sciatic Injection Injury on Enzymohistochemistry of Skeletal Muscle in Rabbit’s Legs,R-332
  10. Flavonoids hydration and antioxidant molecules Quantum chemical study,TQ460.1
  11. Studying on Environmrntal Biomarkers Induced by PAEs,X171.5
  12. Extraction, Isolation and Identification of the Metabolites from Plants of Elsholtzia Splendens and Sedum Alfredii Hance Post-phytoremediation,X173
  13. Different electrical properties of carbon nanotubes covalently modified FAD molecular dynamics simulation,TB383.1
  14. Special anti-TB drug resistance degree Capsule Preparation and Quality Standard,TQ463
  15. Overexpression ANT1 Gene in Vascular Smooth Muscle Cells Induced Apoptosis with Bax/Bcl-2 Expression Relations,R541.4
  16. Study on the Chemical Constituents and Effective Fraction of Rosae Rugosae Flos,R284
  17. The Clinical Research of Qiling Tongfeng Decoctionon on Gouty Nephropathy,R259
  18. Fluorescence Determination of DNA and Electrochemical Behaviors of Based,Q523
  19. Baiyangdian analysis of organic pollutants in water,X832
  20. Active ingredient in tea seed oil separation , identification and utilization of,TS229
  21. Study on the Preparation and the Quality Standards of the Wuhuo Depressurization Dripping Pills,TQ461

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > The Chinese herbal medicine preparations of the production
© 2012 www.DissertationTopic.Net  Mobile