Dissertation > Excellent graduate degree dissertation topics show

Pathogenicity of Newcastle Disease Virus in Ducks

Author: ShiYue
Tutor: KongXianGang
School: Chinese Academy of Agricultural Sciences
Course: Preventive Veterinary Medicine
Keywords: Newcastle disease virus Shaoxing Ducks Mallard Pathogenicity
CLC: S858.32
Type: Master's thesis
Year: 2011
Downloads: 77
Quote: 0
Read: Download Dissertation

Abstract


Newcastle disease (Newcastle disease, ND) a variety of poultry infections and deaths caused by the Newcastle disease virus (NDV), an acute, highly contagious disease. Newcastle disease virus host almost all birds, the traditional understanding of waterfowl are the NDV original host, only latent infection without disease. But since 1997, the emergence of the gene VII NDV, have been led to waterfowl geese and ducks infected with the disease, indicating that NDV pathogenic host range tendency to expand; At the same time, from around the world characterization of healthy wild waterfowl have been isolated Class I group, and gene type VII, type II NDV, waterfowl, especially wild waterfowl in the evolution of the Newcastle disease virus, spread attention again. The mallard is the representative species of wild waterfowl, other domestic ducks except Muscovy ancestors in the genetic sense, the study of different genotypes of NDV pathogenicity mallard understand the characteristics of viral replication, contribute to analysis and speculate role of wild waterfowl in NDV evolution and dissemination of the. In this study, the SPF Shao ducks and mallards animal model of Newcastle disease pathogenicity test. Test strains of Newcastle disease standard is selected strong poison F 48 the E 9 , the vaccine strain La Sota, the wild mallard gene type VII NDV Mallard/CH/HLJ363 / 05 (WB363) and the ducks source the virus Md/CH/GD/1/05 (Md). Virus at different doses, different inoculation experiments duck to observe the incidence of death; collection of experimental duck throat and cloacal swabs and virus isolation to determine the time of detoxification and detox; 4-5 days after infection, acquisition duck tissue organs for detecting viruses The tissue distribution; detection of duck serum neutralizing antibody analysis of seroconversion. Establishment of cohabitation infection duck or chicken, the determination of experimental duck virulence of cohabitation poultry. The results showed that the vaccine strain La Sota from low to high doses of different naturally infected intranasally eye pathway, as well as muscle, intravenous pathway vaccination Shao ducks and mallards are not caused by the experimental duck incidence and death. 28d throat and cloacal swabs collected for testing, only in Shaoxing Ducks intranasal eye pathway 10 6 the EID 50 -dose group challenged 3d swab samples a virus is detected, only a short copy. Necropsy experimental duck, necropsy tissue organs viral replication, the experimental duck monitoring 4 weeks seroconversion rates and antibody titers were lower. Two gene VII Newcastle disease virus strains isolated from wild mallard and muscovy duck the the Mallard / CH / HLJ363/05 (WB363), and Md/CH/GD/1/05 (Md) by simulating naturally infected intranasally eye inoculation of 15-day-old SPF Shao ducks and mallards in the highest dose of 10 8.6 the EID 50 / 0.2ml and the lowest dose group 10 6.6 the EID 50 / 0.2ml group are not lead to morbidity and mortality of the duck, but can detect some experimental duck detoxification; intramuscular, intravenous inoculation experiments duck, duck detoxification, some duck the incidence of death, survival ducks significant seroconversion occurs, and the serum antibody titers were significantly higher than La Sota vaccine challenge group. Intramuscular, intravenous injection of death duck tissue organs can be separated to NDV. The experimental duck attack drug detoxification time is not long, and all the living duck no detoxification, morbidity and mortality, serum neutralizing antibody titer of less than 1:4, but with an infected duck rearing cohabitation chicken The incidence of death. The the NDV standard virulent F48E9 of strains by intranasal eye pathway in 10 2.8 Eid 50 / 0.2 ml of dose, can infect 15-day-old Shaoxing Ducks of 10 5.8 < / sup> EID 50 / 0.2ml dose lethal to 15-day-old Shao duck; same dose, 15-day-old mallard detected viral replication and detoxification, incidence and death. F48E9 muscle strains by intravenous way, can be deadly mallard, can detect virus replication in the organs of dead duck multiple organizations. Cohabitation group mallard can produce neutralizing antibodies, but no detoxification, morbidity and mortality. In contrast, one-month-old the mallard on F 48 E 9 by age factors, original magnification virus solution by the intranasal point eye ways to be vaccinated, can not duck the body efficient replication. The preliminary results show that is widely used for chicken immunization vaccine strain La Sota, Shao duck and mallard body can not replicate efficiently, it is not suitable as the prevention and treatment of duck Newcastle disease vaccine candidate strains. F 48 E 9 strains duck virulence gene VII type NDV higher than the currently popular, but they can be found in the duck body replication, was hidden poison state, the potential impact of NDV spread and mutation. The wild mallard Newcastle disease virus resistance higher than the domestic duck and mallard resistance by the day-old.

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Cloning and Fuctional Study of clp Gene of Xanthomonas Oryzae,S435.111.4
  3. Identification of Newcastle Disease Virus from Chicken and the Genome Molecular Characeristics of HN09-68 and HN09-83 Strains,S852.65
  4. Edwardsiella Tarda Infection on Andrias Davidianus and the Protection of Dead Vaccine at Primary Immune,R392.1
  5. Enhancing Effect of Newcastle Disease Virus in Combination with Chemotherapy for Patients with Advanced Non-small Cell Lung Cancer,R734.2
  6. Isolation and Identification of NDV Local Epidemic Strains and the Comparison of Vaccination Programs,S852.65
  7. Identification and Characteristics of Two Pathogens of Silkworm Bombyx Mori,S884
  8. Studies on the Pathogenic Roles of Sialic Acid in Streptococcus Suis Serotype 2,S858.28
  9. Isolation and Identification of Riemerella Anatipestifer in Weifang Reagion and the Research and Application of Riemerella Anatipestifer Inactivated Vaccine,S858.32
  10. Optimization of Newcastle Disease Immunization Programs,S855.3
  11. Construction of Recombinant NDV-rAnh/EGFP and Investigation of Its Tumor Inhibitory Ability,S852.65
  12. Construction of a Recombinant Newcastle Disease Virus Expressing Rabies Virus Glycoprotein and Research of Immunogenicity,S852.65
  13. The Extraction, the Effects on the Newcastle Disease Virus in Vitro and the Action on Duodenums and Jejunum Mucosal Immunity in Vaccinated Chickens of Opuntia Monacantha Polysaccharides,S858.31
  14. The Effect of Honeysuckble Compound Preparation on the Immune Function in Immune Organ of Chickens Infected with NDV,S858.31
  15. Cloning and Analysis of F Gene and HN Gene of Newcastle Disease Virus ND-xx08 Isolate,S852.65
  16. Identified of Enterococcus Faecalis Isolated from Septicemia Geese and Prokaryotic Expression of Cytolysin Activator,S858.33
  17. Study on the Polymorphism of Anas Platyrhnchos Mstn Gene and Meat Production Performance Relevance,S865
  18. Dynamic Properties of Interaction of Class Ⅰ Newcastle Disease Virus with Chicken Embryo Fibroblast Cells,S852.65
  19. The Genomes of Two Field Ndv Were Sequenced and the Quantity Changes of These Viruses in CEF and Spf Embroyed Eggs During Passages Were Analyzed,S852.65
  20. The Analysis of the Adjuvant Effects of Salmonella Typhimurium Flagellin,S852.5
  21. The Establishment of Minigenome and Full-Length Genome for NDV of Duck Origin of NDV08-004 Strain,S852.65

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Poultry > Duck
© 2012 www.DissertationTopic.Net  Mobile