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Screening and Analysis for Efficient Co-inoculation of Soytean Rhizobia and Plant Growth-promoting Rhizobacteria

Author: XueXiaoZuo
Tutor: FengRuiHua
School: Chinese Academy of Agricultural Sciences
Course: Microbiology
Keywords: Rhizobia Rhizosphere promoting bacteria ( PGPR ) Filter Phosphorous Bacteria Auxin ( IAA ) Dual inoculation Proteomics
CLC: S565.1
Type: Master's thesis
Year: 2011
Downloads: 115
Quote: 3
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Abstract

Rhizosphere growth-promoting bacteria (PGPR) is a class of survival in the plant rhizosphere range, through the secretion of plant hormones and other methods to promote plant growth, or the pathogen antagonism beneficial bacteria in agriculture has application value. Many studies show that, of PGPR were inoculated legumes promote nodulation, including increasing the number of tumors, and promote root development. Rhizobia and other beneficial microorganisms composite promote soybean nodulation nitrogen fixation has become an important content of the soybean Rhizobium applied research. In this paper, the the soybean rhizosphere separation PGPR and the room deposited strain screening promoting bacteria dual inoculation with rhizobia. Vermiculite and soil pot experiment was screened five pairs of soybean Rhizobium nodulation ability with the promotion of the role of promoting bacteria, the excellent combination of soybean rhizobia - promoting bacteria and its mechanism was studied, and the main results are as follows : the qualitative determination of the phosphate solubilization capacity and production of auxin from the tested 73 strains were screened to the a phosphate solubilization capacity strains 24 and 13 of auxin production strains. Of these, nine promoting bacteria and dissolved phosphorus and secretion of growth hormone. Select 14 promoting bacteria with broad spectrum of fast-growing the soybean rhizobia 4644 and slow-growing the soybean rhizobia 4219 vermiculite potted plants inoculated with five excellent combination of dual inoculation of fast-growing soybean rhizobia 4644 and Bacillus bacillus 1.15, promoting bacteria P13 and P7 and slow-growing soybean rhizobia the 4219 Bacillus megaterium 92075 and promoting bacteria 1-3. Compared with the single vaccination, dual inoculation in nodule number, nodule dry weight, shoot dry weight and plant nitrogen content were improved, and better able to promote soybean nodulation and growth. Fuyang, Yan'an and Jining soil potted double vaccination the rescreening experimental results are basically the same pot with vermiculite. But different in different parts of the soil, the increase in value of the five were inoculated with a combination of various indicators. Phosphate solubilization ability of quantitative measurement results showed that, 5 promoting bacteria are dissolved inorganic phosphorus, Bacillus subtilis 1.15 and promoting bacteria P13 phosphate solubilization the strongest, the amount of dissolved phosphorus, respectively 255.58mg / L and 266.81mg / L. Determination of total phosphorus content of the soil potted soybean plants, double vaccination makes the plant phosphorus content increased by at least 10% or more, up to 62.5%. The HPLC Determination promote the raw ingredients of the fermentation broth, results showed that five promoting bacteria produce plant hormones and organic acids. 5 promoting bacteria has a production capacity of auxin. In addition to promoting bacteria 1-3, the rest of the strain does not produce zeatin. Isolated from soybean rhizosphere soil promoting bacteria P7, P13 and promoting bacteria 1-3, the determination of the 16S rDNA sequences were with Phyllobacterium ifriqiyense Sinorhizobium meliloti and Bacillus firmus has 99% homology, attributable to the leaves bacilli the genus (Phyllobacterium sp.), China Rhizobium (Sinorhizobium sp) and the genus Bacillus (Bacillus sp.) Proteomics promoting bacteria to improve the soybean Rhizobium nodulation the molecular mechanism of nitrogen-fixing capacity. Filter from the growth-promoting strains in promoting bacteria P7 and fast-growing soybean rhizobia 4644 were inoculated to promote its nodulation and nitrogen fixation, Pseudomonas fluorescens 10040 and strain 4644 were inoculated with no such effect. Promoting bacteria P7 fermentation broth and Yellow 13 daidzein processing strain 4644, by two-dimensional electrophoresis and mass spectrometry analysis to determine the response of the protein, found 22 up-regulated protein, 4 down proteins involved in glucose metabolism of protein and a lot of assumptions proteins. Pseudomonas fluorescens 10040 fermentation broth and yellow 13 daidzein processing strain 4644 found 60 up-regulated protein, 14 down-regulated protein involved in the metabolism of protein, DNA replication transcription-associated protein and beta-lactamases. Most of the rest of the exhibit differences in protein in the database can not retrieve matching information.

Screening and Analysis for Efficient Co-inoculation of Soytean Rhizobia and Plant Growth-promoting Rhizobacteria

Abstract

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