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Soybeans are the world's major crops by Phytophthora sojae (Phytophthora sojae Kaufmann Gerdemann.) Caused by Phytophthora root rot is second only to soybeans, the second largest soybean cyst nematode disease, seriously affecting the world's soybean production. In China, in the northeast of Phytophthora sojae, Fujian and other places cause serious harm. In this study, China and the United States, a group drawn from the resources of soybean Phytophthora resistance gene is derived, and which may contain Rps1 seat soybean germplasm resistance genes by molecular testing. In addition, on 15 soybean varieties Wandou contained Phytophthora root rot genes genetic mapping. The main results are as follows: 1. Use hypocotyl inoculation method identified 156 domestic soybean resources 13 resistant strains of Phytophthora sojae and conducted genetic derivation. The results show that there are at least 125 parts of soybean germplasm resistant strains of P. sojae one, accounting for 80.13%. This produced a total of 125 parts of soybean resources 90 different reaction type, which has 10 reactive meet a known single gene, one or more of the two genes combined reactive inferred these resources contain Rps1a, Rps1b, Rps1d, Rps1k , Rps3a, Rps3b, Rps3c, Rps4, Rps5, Rps6, Rps7 and Rps8 other genes. 2 Use the hypocotyl inoculation method cited in 85 parts of the U.S. soybean germplasm for 13 strains of P. sojae resistance identification, combined with gene derived pedigree analysis done. The results showed that there were 72 identified anti 1-12 sojae strains. And pedigree analysis through gene derived clear 35 varieties (lines) with known resistance genes or gene combinations, which has 14 varieties (lines) containing Rps1a, 1 δΈͺ containing Rps1c and 2 contain Rps1k, these three genes can effectively resist China's soybean Phytophthora species, can be directly used for breeding for disease resistance. 3 alleles seat use and Rps1 SSR markers tightly linked to the gene derived allele containing Rps1 seat 41 soybean resources for testing. Found L60-246 (Clark63), L72-1138, L63-1612, L72-1140, L83-4387, Maple Arrow, Dawson, Wilkin, Hodgson 78, Vinton 81, Beeson, L81-4871, Century lx13, Hoyt, Dunbar, Delsoy4710, L72D-4045, L88-8739, L69-4428, Kato, Hack and Jindou the 15th and 22 parts of soybean germplasm containing Rps1a; L82-1858 contains Rps1c; Qi Huang 1, Wen Feng 7, No. 1 soybean Stone , New Section 4, Monte 9793-1, Jindou 31, Ludou 4, Cat beans (ZDD18835), the new six Yudou 12 Green and 10 parts of soybean and other resources containing Rps1d; L81-4590, Sprite 87, Amcor 89 , L87-0482, Hobbit87 and 7651-1 resources such as soybeans containing 6 parts Rps1k. 4 Wandou 15 varieties of soybean resistance gene RpsWD15 Phytophthora sojae plotting. And its positioning in D2 linkage group, SSR markers Satt514 and Satt543 RpsWD15 are located on both sides of their genetic distances were 4.8 cM and 3.1 cM.
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