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Studies on Effect of Various Cultivars and Secondary Metabolites of Tea Plants to Tea Green Leafhopper Feeding by DC-EPG

Author: LiuLiFang
Tutor: HanBaoZuo
School: Chinese Academy of Agricultural Sciences
Course: Agricultural Entomology and Pest Control
Keywords: Empoasca cicadas Tea Varieties Secondary metabolites DC-EPG Sucking behavior Cluster analysis Crop insect resistance
CLC: S435.711
Type: Master's thesis
Year: 2011
Downloads: 59
Quote: 2
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Abstract


False-eye leafhopper Empoasca vitis G? The tea-growing areas of the Chinese mainland harm the most serious pests. Production often found tea tree are victims of different varieties of different phenomena, but this lack of quantitative research; tea plant secondary metabolites material is the material basis of the tea plant resistance to insects, the the various metabolites anti cicada rarely reported. This article uses visible insect feeding behavior observation instrument that insects spying potentiostat (DC-EPG) to study the feeding behavior of leafhoppers in 6 kinds of Jiangsu, Zhejiang tea-growing areas on the main push varieties, longer times to the nine categories tea phloem biomass as food source, they are detected DC-EPG of Empoasca cicadas feeding behavior, draw the following test results: 1. analysis Jiangsu, Zhejiang Tea District main push of the six varieties of tea on leafhoppers take Fresh results found: ① leafhoppers in six tea varieties on seven kinds of basic sucking waveform is the same, that is, non-feeding wave NP wave spying wave A wave saliva secretion wave S wave, stylet arrive before the phloem active the feeding wave C wave passive phloem feeding wave E wave, stylet within the outer membrane or extracellular cell wall mechanical puncture blocked waveform F wave, R wave of the rest wave. ② in tin tea on the 5th, the the spying number of Su tea less long, of passive phloem feeding wave E wave duration; the spying in Longjing 43 times, the passive feeding in the phloem wave E wave short duration; Su tea 120, in the tea 108 number and E-wave duration Anjibaicha spying center. (3) is generally believed that the spying fewer E wave lasted long, sensual strong. The use of cluster analysis, multiple comparisons judge six tea varieties resistance to leafhopper feeding capacity from strong to weak order: Longjing 43 gt; Su tea 120 gt; in the tea 108 gt; Anji white tea gt; Su tea gt; the the leafhopper population density of small to large tin tea on the 5th, the order field six years of observation tea varieties order is basically the same. DC-EPG technology recorded the feeding behavior of the leafhopper in the nine categories tea secondary substances significantly following waveform: passive phloem feeding wave E wave, spying wave the np-wave, to spying wave A wave , the salivation wave S wave. E-wave np wave, and paths wave (AS) as an indicator cluster analysis these nine substances into three categories. E-wave combined with np wave lasted: theaflavins promoting leafhopper feeding; theanine, chlorogenic acid, glutamic acid, gallic acid catechin EGCG and ECG is resistant to leafhopper feeding; tea phenol, catechin and leafhopper feeding behavior of low correlation. Compared with the control (5% sucrose solution) 0.05% theaflavins for leafhoppers role in promoting a strong feeding (p lt; 0.05); glutamic acid, chlorogenic acid and EGCG concentration of 0.15%, Theanine, ECG and EGC 0.10% leafhopper has refused spying and antifeedant effect (p lt; 0.05). Analysis: DC-EPG technology can be used to quickly judge the different varieties of tea tree leafhopper resistance as varieties for leafhopper resistance auxiliary means of detection. DC-EPG technology can be used to compare allelochemicals leafhoppers palatability leafhopper feeding activity, which is anti-Tea different varieties contain a significant difference in the quality and quantity of secondary substances, may to some extent explain the tea tree different species of leafhopper resistance or sensibility.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Beverage crop pests and diseases > Tea pests and diseases
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