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Plants during growth of pathogenic microorganisms will be infested with these pathogens in the long struggle of co-evolution process of gradually formed a series of complex and effective defense mechanisms to protect itself. Systemic acquired resistance in plants resistant to pathogen attack complex defense mechanisms, and the resistance of the signal substance in the defense processes play an important role. This paper studies the number of signal substances in plant disease resistance enrichment and detection methods, and studied in Pseudomonas solanacearum infection of peanut plants under salicylic acid, jasmonic acid and other signal substances in the variation of the response, the findings are as follows: 1. Designed and assembled dynamic adsorption unit, and high performance liquid chromatography - tandem mass spectrometry for the determination of trace peanut plant volatiles - methyl jasmonate and methyl salicylate qualitative and quantitative methods. Adsorbent using Tenax-TA volatiles, 0.1% formic acid - methanol eluate was concentrated sample backward. The column was Agilent C18 column (2.1 × 150 mm, 5μm), methanol and 0.05% formic acid - water as mobile phase, flow 300μL/min, gradient elution. With a triple quadrupole mass spectrometry / tandem linear ion trap, using multiple reaction monitoring (MRM) positive ion mode determination, internal standard. Method is simple and quick, enriched, high efficiency, two kinds of volatiles detection limits (S / N = 3) were 2.0μg / L and 20μg / L, the limit of quantification (S / N = 10) were 5.0μg / L and 50μg / L; in 1.0-1000μg / L when the peak area ratio and good linear relationship between the mass concentration (r gt; 0.98). (2) the use of solid-phase extraction and high performance liquid chromatography - tandem mass spectrometry, determination of peanut leaves three signals substances, salicylic acid, jasmonic acid and methyl jasmonate. Use of alcohol / water / concentrated hydrochloric acid (volume ratio of 2: 1: 0.002) and dichloromethane extract of peanut leaves salicylic acid and jasmonic acid, 80% methanol solution of methyl jasmonate leaf extract, and purified through a C18 column After concentration, the final use to detect HPLC-MS/MS. Methanol and 0.05% formic acid - water as mobile phase, using multiple reaction monitoring (MRM) positive ion mode determination, external standard. Method to extract high efficiency, good linearity and reproducibility, detection limits for several compounds 0.005-0.5 between methods in 67.03% -103.34% recovery. 3 peanut plants as the material, the use of previously developed method, the detection of Pseudomonas solanacearum infection under 0,3,6,9,12,24,48 h after the peanut plant leaves salicylic acid, jasmonic acid and jasmonic methyl changes. Peanut plants of volatile methyl jasmonate signal substance and the change of methyl salicylate was measured in the same manner. The results showed that R. solanacearum infection can cause signal substances in these plants increased. This also proves that plants after the injury, the injury site in the original signal molecules produced by some of the body's defense system startup. The study also found that in resistant varieties in vivo signal salicylic acid, jasmonic acid and methyl jasmonate maximum time earlier than the susceptible cultivar 3 h, resistant varieties described the response to injury more rapidly, initially revealed resistant varieties and susceptible plants within the response to pathogens infect different species of resilience for the future development of new mining and non-polluting, high-efficiency ecological pesticide provides a guideline.
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