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With the massive planting of transgenic technology development of transgenic crops, genetically modified crops may bring negative impact on human health, biodiversity and environmental safety also gradually attracted national attention. To this end, the European Union, Japan and other countries began to implement mandatory labeling system for genetically modified crops, specificity, and accuracy of the GMO detection technology proposed stringent requirements, GMO detection technology can also become a hot topic. China on March 20, 2002 implementation of the \Soybean four crops. Therefore, the establishment of a set of convenient shortcut GMO detection technology is an important prerequisite to implement the labeling system. In this study for the detection of genetically modified crops in four categories, multiplex PCR primer design method to establish the subject, establish a set of four multiplex PCR detection system, the main results obtained are as follows: 1, soybean endogenous gene Lectin exogenous anti-herbicide gene EPSPS CaMV35S promoter and the NOS terminator for the detection of objects, four pairs of primers were designed to establish, by studying the better end of the primer concentration ratio and annealing temperature, soybeans genetically modified quadruple PCR detection system . The results showed that establish quadruple PCR system for the simultaneous detection of endogenous genes and genetically modified ingredients, good stability. Corn within the gene IVR exogenous herbicide-resistant gene (PAT), insect-resistant genes, the Cry1Ab screening gene NPT II CaMV35S promoter and the NOS terminator target fragment detection through research better final primer concentration ratio and the annealing temperature, the establishment of a Seventh corn genetically modified PCR detection systems. The results show that the established sept PCR system for the simultaneous detection of the endogenous gene and GMO is feasible, the detection method, high efficiency, good stability. 3, the PEP to rape endogenous gene, herbicide-resistant gene (BAR, PAT), screening of gene the NPT Ⅱ, common promoter (CaMV35S FMV35S NOS) terminator for the detection of objects through research better primer final concentration ratio and annealing temperature, the of rapeseed meal GMO multiplex PCR detection system. The results show that the detection system established in this study can effectively detect rapeseed meal as well as other crops (soybeans, corn, rice, cottonseed) GMO detection process is simple, accurate, and should be widely applied. 4 cotton endogenous genes sad1, reporter gene GUS the Foreign Genes Cry1Ab/Ac, screening gene NPT II NOS terminator promoter and CaMV35S promoter for the detection of objects, study better primer final concentration ratio and annealing temperature. 6 heavy cotton genetically modified PCR detection systems. The results show that: cottonseed genomic DNA extraction methods established in this study, the six re-PCR detection systems can be effective from as little as a small sample of cottonseed detected in the genetically modified cotton. This study, multiplex PCR detection process through exploring establish a set of convenient, practical effect of the fine multiplex PCR primer design system, this multiplex PCR primer design methods by the primers verification has as many as 45, and several primers adjust the test, to overcome the difficulties on multiplex PCR primer design, there is a good reference. Multiplex PCR detection system for the study of the four crops genetically modified ingredients, repeated validation transgenic crops developed multiplex PCR detection kit to good effect, suitable for promotion and application, and can be used as a rapid detection of transgenic plants and their products GMO's optional.
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