|
Kidney cancer (carcinoma of kidney), also known as renal cell carcinoma, renal adenocarcinoma, originated in tubular epithelial cells, can occur in any part of the renal parenchyma, is a high rate of fatal malignancy of the urinary system. Newly issued annually with the world and domestic renal cell carcinoma cases and deaths is rising, the morbidity and mortality increase every year, the study of the pathogenesis of renal cell carcinoma to seek more accurate and efficient diagnosis, thus effectively services in the judgment of the treatment and prognosis of renal cell carcinoma, more and more attention. More and more in-depth study of the pathogenesis of renal cell carcinoma, many molecular markers associated with renal cell carcinoma has been found to provide the theoretical basis for molecular targeted therapy for renal cell carcinoma. Adrenomedullin as a vasoactive peptides, in the generation of tumor cell invasion and metastasis process have become more attention. Adrenomedullin (adrenomedulin, ADM) is an autocrine growth factor in human tumor tissue, and many studies show that is widely distributed in various types of tumor tissue or tumor cells, by inhibiting the immune response, inhibition of apoptosis, and promote angiogenesis stimulate mitosis and other mechanisms play an important role in the occurrence, development and metastasis of tumor process. Objective: This study was detection of renal cell carcinoma, ADM and ADMR expression in normal kidney tissue, and to explore the role of ADM and ADMR in renal cell carcinoma and development process, and the study of renal cell carcinoma to generate growth and invasion transfer of molecular mechanisms to provide a new molecular markers for prognosis and staging, treatment for renal cell carcinoma, especially molecular biology, targeted therapy provide a theoretical basis. Method: collect data integrity cases of renal cell carcinoma, 33 cases (April 2009 to September 2009). The specimens were immediately drawn under sterile conditions in postoperative specimens including cancerous tissue from the tumor margin 2cm above normal renal parenchyma. Immunohistochemical staining and flow cytometry methods were applied to detect the expression of ADM and ADMR renal clear cell carcinoma and normal kidney tissue, and correlation analysis ADM and ADMR In the expression in renal cell carcinoma. Results: Immunohistochemical detection of ADM expression in renal cell carcinoma was 100%, ADM expression in normal kidney tissue weakly positive, the positive rate of 9.1%; flow cytometry method to detect renal tumor tissue ADM expression levels for 1267.84 ± 127.52, 970.36 ± 171.46, p = 0.000 lt; level of 0.05, ADM expression in renal cell carcinoma was significantly higher than ADM ADM expression levels in normal renal tissue level of expression in normal kidney tissue. ADMR protein expression level of the renal cell carcinoma 1161.91 ± 202.71, normal renal tissue ADMR expression level of 826.05 ± 171.99, p = 0.000 lt; 0.05, renal cell carcinoma, the expression level was significantly higher than ADMR expression in normal renal tissue level. Bivariate correlation test, ADM and ADMR correlation coefficient of 0.929, P = 0.000 lt; 0.05, ADM and ADMR expression in renal tumor tissue were positively correlated. Conclusion: ADM and ADMR in renal tumor tissue was significantly higher than normal renal tissue expression, and ADM and ADMR In the renal tumor tissue expression was a positive correlation, indicating that ADM and ADMR in the occurrence of the tumor cells, the development process play a role in promoting, ADM will likely become a new target for molecular targeted therapy in renal tumors.
|