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Study on Mechanism of Inflammatory Reaction of Dendritic Cells in Rats after Intracerebral Hemorrhage

Author: YangZuo
Tutor: LiXiaoGang
School: Luzhou Medical College
Course: Neurology
Keywords: Dendritic cells Cerebral hemorrhage Glial cells Antigen-presenting Immune response
CLC: R743.34
Type: Master's thesis
Year: 2011
Downloads: 22
Quote: 0
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Abstract


Objective: To detect rat caudate nucleus cerebral hemorrhage model by establishing different time points perihematoma cerebral edema, the relationship between changes in the expression of leukocyte interleukin -6, GM-CSF and OX62 OX42 positive cell count, and then explore the dendritic cells after cerebral hemorrhage secondary sources and mechanisms in the central nerve injury. Method: an autologous blood injected into the rat brain caudate nucleus produced cerebral hemorrhage model. 2 50 SD rats were randomly divided into two groups: sham group, ICH group, n = 25. Each group of five observation time points were postoperative 24h, 48h, 72h, 5d, and 7d, five mice per time point. 24h after successful evaluation of the model, at each time point after nerve dysfunction score. Wet and dry weight determination of the water content of the brain tissue at different points around the hematoma. Enzyme-linked immunosorbent assay detection of rat brain homogenates, IL-6 and GM-CSF levels. Using immunohistochemistry and microscopy to observe changes in dendritic cells and glial cells, the number of each group at different points around the hematoma. Results: 1. Sham group at each time point did not cause neurological dysfunction; neurological dysfunction in the ICH group 48h of the most serious, and then gradually improve the ICH group compared with the sham group, there is a statistically significant difference at each time point (P lt; 0.05), the severe neurological disorder. 2.24h brain water content of cerebral hemorrhage group and the sham group there is no difference (P gt; 0.05), the water content of the ICH group then began to increase 48h-72h peaked compared with the sham group was statistically significant (P lt began to decrease; 0.05), 72h, postoperative 5d and 7d still increased control group, but no significant differences (P gt; 0.05). 3. The hematoma surrounding tissue IL-6 expression in 24h elevated ICH group than in the sham group, a statistically significant difference (p lt; 0.05), peaked at 48h, then decreased postoperative 7d two groups of rat brain tissue. IL-6 expression is no longer statistically significant difference (P gt; 0.05); pulp GM-CSF in the two groups of rat brain tissue homogenates in 24h statistical difference (P gt; 0.05). Elevated expression of GM-CSF 48h ICH brain tissue, a statistically significant difference (p lt; 0.05) compared with the sham group, 48h-7d continues to rise each observation time point of interest learning differences (p lt; 0.05 ). 4.ICH group OX42 OX62 cells and the number of glial cells at each time point were significant changes in 24 hours when the ICH group than in the sham group glial cells began to increase the number of statistically significant (P lt; 0.05), and over time the extension continues to increase 7d peaked statistically significant (P lt; 0.05); 24h OX42 OX62 cells for ICH group compared with sham group began to increase the number of statistically significant (P lt; 0.05), and with the extension of time continues to increase, the 7d peaked statistically significant (P lt; 0.05). Correlation analysis: ICH group of OX42 OX62 cell number change and GM-CSF expression was positive related changes (R gt; 0 p lt; 0.05); ICH group of OX42 OX62 cells and OX42 glial cell number of changes were positively correlated with changes (R gt ; 0 p lt; 0.05); ICH brain tissue pulp IL-6 content and hematoma surrounding brain tissue water content was positively related to change (R gt; 0, p lt; 0.05). Conclusions 1. autologous blood production in rats cerebral hemorrhage animal model, create a simple, inexpensive, pathophysiological changes consistent with cerebral hemorrhage, cerebral hemorrhage as widely used animal models .2. observed after ICH the hematoma surrounding tissue in glial cells may is the main source of DC one. The 3. Perihematoma DC depending on the environment conditions. In early hematoma formation may promote inflammation, increased brain damage; the hematoma late suppress the immune response, and promote the repair of damaged tissue.

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CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke) > Cerebral hemorrhage
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