|
Objective: To observe the Guanyin mixture anorexia (lung spleen deficiency syndrome) in children and model of rat plasma VIP explore the efficacy and mechanism of the the Guanyin agent treatment of infantile anorexia, lay the foundation for the development of new drugs and research. Methods: 1. Experimental study: healthy SPF SD rats were randomly divided into five groups, Guanyin mixture of low-dose group, Guanyin mixture of high-dose group, the control group the (SHENLINGBAISHU mixture group), model group and blank group 12 in each group. The blank group to give regular diet, the other four groups Wang by pass simulating the cause made specially diet for 3 weeks. After the modeling, the start of treatment, three weeks of continuous treatment. Changes in food intake and body weight of each group after the end of the observation and treatment, and blood was collected from the abdominal aorta in rats using RIA detection technology determination of the concentration of rats plasma VIP (Vasoactive intestinal peptide, VIP). Clinical research: select the outpatients lung spleen deficiency syndrome and 42 cases of anorexia in children were randomly divided into two groups, the treatment group and 22, oral Guanyin mixture control group (n = 20), oral Shenling Atractylodes mixture, were treated for 1 months. Observed the syndrome changes before and after treatment, respectively before treatment, after mining the morning fasting blood using radioimmunoassay Determination of plasma VIP concentration. Results: 1. Experimental part: the parties after the success of the module in modeling, body weight and food intake decreased significantly compared with the control group the difference was statistically significant (P lt; 0.01); concentration of plasma VIP model group and blank group difference was statistically significant (P lt; 0.05). Plasma VIP concentration compared with the model group decreased after treatment by Guanyin mixture, the low-dose group compared with model group differences were statistically significance (P lt; 0.05, P lt; 0.01), and the high-dose group closer to the control group, The low-dose group and positive group had fallen to the level of the control group, but no statistically significant difference compared with the control group. 2 clinical part: treatment group recovered in three cases, 14 cases markedly effective in 4 cases, and 1 case, the total effective rate of 63.64%; control group healed 0 cases, 8 cases were markedly effective in 11 cases, and 1 case, the total effective 40.00%, two groups of diseases efficacy comparison, the difference was statistically significant (P lt; 0.05), the treatment group than the control group. Two sets of TCM syndromes compared treatment group was 68.18%, the effective rate is 40.00% of the control group, the difference was statistically significant (P lt; 0.05), the treatment group than the control group. After treatment, TCM climate integral than before treatment decreased the difference was statistically significant (P lt; 0.01), but the integral of the treatment group decreased more significantly between the two groups, the difference was statistically significance (P lt; 0.05). Plasma VIP concentration after treatment than before treatment decreased the difference was statistically significant (P lt; 0.01), but more significant decline of the treatment group compared with the control group, the difference was statistically significance (P lt; 0.05 ). Conclusion: 1. Guanyin mixture can improve the syndromes of anorexia (lung, spleen deficiency syndrome), to enhance the research object appetite, increase their food intake, and more effective than SHENLINGBAISHU mixture. Guanyin mixture can reduce the study of plasma VIP concentration reduction is greater than Mixture of SHENLINGBAISHU. Guanyin mixture in treating anorexia (DLS certificate) initially speculated mechanism regulating peripheral blood VIP levels.
|