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Objective To investigate the proliferation of changes in human fetal cardiac fibroblasts (HFCFS) intervention by the different concentrations of aldosterone (ALD) , and cell type Ⅰ, Ⅲ collagen expression to find and explore the related signaling molecules aldosterone induced myocardial fibrosis . The Methods collagenase II digestion , differential adherence method the differential off wall obtained and purified HFCFS , the effect of different concentrations of aldosterone , application of CCK-8 live cell counting kit to detect cell proliferation rate . By RT -PCR method to detect changes in the cells of collagen type Ⅰ before collagen A1 ( of COL1A1 ) and collagen type Ⅲ before collagen A1 ( COL3A1 ) mRNA in the , application Westem blot detection CoL1A1. COL3A1 protein expression changes , the human genome expression microarray detection of expression differentially expressed genes . Results ALD in a concentration -dependent promote HFCFS proliferation ; within a certain range of concentrations ( 10-9,10-8,10-7 mol / L ) , ALD can significantly promote HFCFS expression in COL1A1.COL3A1 , while the 10-6mol / L of ALD no apparent effect , 10 mol / L of ALD even play an inhibitory ; the chips screened CCL2, DAAM1, POLR2A, SDC1, HSPA1A, CLDN14 differentially expressed genes . Conclusions ALD can promote the cell proliferation of HFCFS , the extent of the proliferation of elevated concentration in a certain concentration range ; ALD can promote expression of COL1A1 and COL3A1 , ALD high concentrations inhibit the expression of two molecules . The aldosterone intervention , HFCFS the proliferation of COL1A1 , COL3A1 mRNA and protein expression is not completely was a linear relationship . Aldosterone myocardial fibrosis complex signal transduction pathway of CCL2 , MAKP , Wnt , and other signaling molecules may play an important role .
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