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The papers on clustered along the silk umbrella Naematoloma fasciculare fermentation process, the chemical composition of its fermentation products and fruiting, antitumor and antibacterial activity in vitro study. Clustered along the silk umbrella fermentation process, submerged culture mycelia and secondary metabolite research, filter out the most suitable carbon and nitrogen sources and inorganic salts, to medium formulations optimized by the response surface method clustered along the silk umbrella optimal fermentation process medium formula, maltose 59.27g / L, peptone: yeast powder = 1:1 to 8.04 g / L, potassium dihydrogen phosphate: magnesium sulfate: ammonium sulfate = 1:1 : 2.27 g / L, placed in a water bath oscillator, speed 120r/min, temperature of 25 ° C, shaking culture 9d biomass of up to 15.06 g / L. This medium formula more Yoshihito Shiono such as al formula is more reasonable, a more balanced mix of carbon, nitrogen and inorganic salts, inorganic salts than nitrogen source point of view and from the analysis of the Box-Benhnken test results on clustered along the wire Agaricales silk accumulation of inorganic salt is indispensable in the cultivation of mycelium, another clustered along the silk umbrella of the organic nitrogen utilization was significantly better than inorganic nitrogen. Clustered along the silk umbrella fermentation on mice LDso value of 3.15 g / kg. Chemical composition, clustered along the silk umbrella fermentation products of petroleum ether extract and fruiting bodies of petroleum ether extract by GC-MS analysis shows that clustered along the silk umbrella fermentation products and fruiting petroleum ether extract chemical composition is closer. Fascicled along the wire umbrella fruitbody reflux extraction, the ethyl acetate extract was purified by column chromatography on chemical components were separated, to obtain a total of five of the monomer compound, by electron bombardment mass spectrometry or high resolution mass spectrometry, NMR spectroscopy, 13C-NMR, 'H-NMR NMR spectroscopy, DEPT-90 and DEPT-135 NMR spectroscopy methods such as identification of compounds 1-5, respectively, 1,3 - dione -4 - dimethyl -10,13,14 - trimethyl -15 - hydroxy-17 - (1 - methyl-2 ,3 - heptenoic acid) -8,9,11,12 - diene-tetracyclic triterpenoid, 2,3 - dihydroxy-4 - dimethyl - 10,13,14 - trimethyl -17 - (6 - hydroxy-4 ,5 - heptenoic acid) -8,9,11,12 - diene tetracyclic triterpenoid, 4 ', 5 - dihydroxy -7 - methoxy flavanone (aka cherry kiosk), 4,6,8 (14), 22 (23) - tetraene -3 - ketone ergost alkoxy and fasciculolB of the compounds 1-4 for the first time from the clustered along silk umbrella fruiting bodies were isolated. Antitumor H22 tumor clustered along the silk umbrella fermentation material dose of the investigational drug were 200mg/kg, 400mg/kg, 600mg/kg, 800mg/kg, 1000mg/kg, inhibitory rate was calculated, spleen index, liver index, thymus index, white blood cell count and IL-2 levels were compared with the saline control group and CTX group, evaluation clustered along the the silk umbrella fermentation was anti-tumor activity. Based on experimental data statistical seen clustered along the silk umbrella fermentation products the dose 200mg/kg, the H22 tumor inhibition rate of up to 54.71%, and the spleen index, thymus index, liver index, white blood cell count and IL-2 content compared with the control group had varying degrees of improve, spleen index and IL-2 content with the control group compared showed a significant difference (P lt; 0.05), thymus and liver index and CTX group comparison showed a significant difference (P lt; 0.05), white blood cell count, compared with the control group showed a highly significant difference (P lt; 0.01), CTX group comparison showed a significant difference (P lt; 0.05). Clustered along the silk umbrella fermentation product dose to 400mg/kg, 600mg/kg 800mg/kg, inhibition rates were 30.38%, 26.44%, 10.65%, and the spleen index, thymus index, liver index, white blood cell count and IL- 2 content compared with the control group had varying degrees of decline. Clustered along the silk umbrella fermentation product dose 1000mg/kg of H22 tumor inhibitory rate of -72.17%, and the spleen index, thymus index, liver index, white blood cell count and IL-2 levels were lower than control group. fasciculolB no inhibitory effect on the proliferation of SMMC-7721 cells. In vitro antibacterial extract, clustered along the silk umbrella fruiting bodies by reflux extraction, using the disk diffusion method vitro antibacterial activity of the test statistics, we can see, clustered along the silk umbrella ethyl acetate extract 37mg / ml, the highest inhibition rate of E. coli ATCC8099, 36.59%, and the positive drug group showed a highly significant difference (p lt; 0.01). Clustered along the silk umbrella petroleum ether extract 37mg/ml, Staphylococcus aureus ATCC6538 highest inhibition rate was 84.38%, with positive drug group was highly significant difference (p lt; 0.01). Clustered along the silk umbrella chloroform extract concentration of 37mg/ml, Bacillus subtilis ACCC11060 highest inhibition rate to 39.36%, with positive drug group was highly significant difference (p lt; 0.01). Agrobacterium EHA105 highest inhibition rate when clustered along the silk umbrella petroleum ether extract 18.5mg/ml 35.38%, with positive drug group was highly significant difference (p lt; 0.01). The compounds fasciculolB concentration of 3 × 10-2mg/mL, Staphylococcus aureus ATCC6538 antibacterial rate of 12.27% concentration of the 6 × 10-2mg/mL, the inhibition rate of 0.58%. 2,3 - dihydroxy-4 - dimethyl -10,13,14 - trimethyl -17 - (6 - hydroxy-4 ,5 - heptenoic acid) -8,9,11,12 - diene tetracyclic triterpenoid concentration for the 3 × 10-2mg/mL rate of 0.19% of Staphylococcus aureus ATCC6538 antibacterial. The compound 1,3 - dione -4 - dimethyl -10,13,14 - trimethyl -15 - hydroxy-17 - (1 - methyl-2 ,3 - heptenoic acid) -8,9,11 , 12 - diene tetracyclic triterpenoids concentration of 3 × 10-2mg/mL antibacterial activity against Staphylococcus aureus ATCC6538 rate of 25.61%, Agrobacterium tumefaciens EHA105 inhibitory rate of 32.98%, the concentration of the 6 × 10-2mg/mL against Staphylococcus aureus ATCC6538 inhibition rate of 14.90%, Agrobacterium tumefaciens EHA105 inhibition rate of 12.96%. Clustered along the silk umbrella of Candida albicans JLC31680 and JLC31681 no inhibitory effect.
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