|
The cocklebur grass Asteraceae Xanthium dried aerial parts of the plant cocklebur Xanthium sibiricum Patrin ex Widder. Slightly cold, bitter, acrid, a small drug. Expelling wind and heat, the efficacy of the insecticide detoxification. Were contained in multiple places in Sichuan Province, Jiangsu Province, Shanghai and other Chinese herbal medicines quality standards, the cocklebur medicinal resources are extremely rich, folk medicine has a long history. Cocklebur roots, stems, leaves, flowers, fruits are used to treat a variety of diseases. Over the years, the fruit surrounding the Cocklebur with involucre Xanthium lot of research work has been carried out. In order to better development and utilization of Xanthium grass, updated to improve its existing local quality standards of Chinese herbal medicines, cocklebur Xanthium sibiricum Patrin ex Widder aerial parts pharmacognosy, including a literature review, commodity survey form histology, TLC identification, assay, fingerprints and pharmacological effects. The results are as follows: Xanthium grass on the original plant to plant morphology, leaf shape, inflorescence position, the shape of the fruit, the density of the involucre spiny beak thorn long or not its main distinguishing characteristics. Stem surface vertical stripes and scattered spots, leaf shape, inflorescence position on the medicinal properties of its main distinguishing characteristics. Organizational structure: stem mainly from the cortex, collenchyma, secretory cavities, clusters of calcium oxalate, vascular type of identification; leaf tissue from glandular hairs, fence, spongy tissue, vascular bundles identification of the type and leaf stomata: powder mainly to leaf epidermal debris, non-glandular hairs, hairs, fibers beam, catheter, wood parenchyma cells, calcium oxalate crystal clusters, etc. as the main distinguishing characteristics. Xanthium grass the TLC identification methods, the results show the different origin of the herbs in the herbs that corresponds to the location of Xanthium grass control was the same color spots, spot color strength of prompt content differences. 12 batches of different origin Xanthium grass samples moisture, total ash, acid insoluble ash check formulate Xanthium grass herbs in water not more than 14.0%, based on the experimental results, the temporary, not more than 17.0% of total ash, acid insoluble ash Not more than 2.0%. Xanthium grass extract measurement method, 12 batches of samples extract content of 11.21% -30.58%, tentatively scheduled to extract content of not less than 20.0%. UV-VIS method for the determination of total phenolic acid method Xanthium grass, the results show quite different Xanthium grass of different origin in the total phenolic content of 0.921% -5.039%, which may be different with herbs growing environment the external environment of the soil, local climate conditions and harvest period certain. HPLC fingerprint of different origin Xanthium grass preliminary total of Xanthium grass HPLC fingerprint mode, a total of 12 peaks. Different harvest periods Xanthium grass moisture check, extract and determination of total phenolic acid content, which leaching, and total phenolic content of the highest the August harvest herbs samples were 28.40%, 3.731%, so Xanthium grass optimum harvest period for an initial period of the end of July to mid-August, summer, autumn flowering or mining cede territory on part with tender fruit. Pharmacological effects Xanthium grass safety evaluation of the experimental results show that the man-day dosage range of 6-9g; the cocklebur grass water extract significantly inhibited xylene-induced ear edema in mice, indicating its Xanthium grass water extract and acetone extract on 104-105CFU/ml amount of bacteria Pseudomonas aeruginosa, Escherichia coli, the duck Salmonella, Staphylococcus aureus, Staphylococcus epidermidis in vitro antibacterial Experimental results show has good anti-inflammatory effects; cocci inhibition is different, and the inhibitory effect of the water extract stronger than acetone extract. Cocklebur Xanthium sibiricum Patrin ex Widder dried aerial parts of the system pharmacognosy, to provide a scientific basis for the the Sichuan Chinese Materia Medica Standards \herbal quality, stable, controllable, and thus ensure the safety of clinical medicine, effective.
|