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Innermongoliansis (S. innermongoliansis) is a native of Inner Mongolia Soda Lake , high quality , medium temperature adaptive spirulina , but found later , special growth conditions and other reasons , such as its basic research in molecular genetics is lagging behind . Improve its heat resistance breeding to expand the scope , reduce farming costs, actual production has great application value. Studies have shown that heat shock proteins in algae resistance to high temperature stress plays an important role . In this study, we cloned by PCR innermongoliansis HSP family three genes : HSP70, HSP90 and DnaJ2, on the gene and its encoded protein sequence and evolutionary relationships of a preliminary analysis indicates that the purpose cloning success. Cloned from Spirulina innermongoliansis p- phycocyanin gene promoters BetaP, which is a constitutive spirulina its strong promoter , the experiment proved the role of a strong promoter . In pBLUNT as the starting plasmid construct conjugative transfer of three expression vectors and select strong constitutive promoter BetaP, the gene 5 'end of the SD sequence (GGAGAG), and adjust the SD sequence and the initiation codon ATG distance of 6 nucleotides, connection gene DnaJ2. Three conjugation in E. coli expression vectors were successfully expressed , expression vector pACYC-BB successfully constructed. Followed by a series innermongoliansis transformation experiments , conjugative transfer into the three conditions were explored and accumulated some valuable experience. Currently, innermongoliansis transgenic research is still in its infancy, the present study can be relevant for future research innermongoliansis provide the basis and reference.
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