Dissertation > Excellent graduate degree dissertation topics show

Cloning and Preliminary Function Analysis of a Female Gamete Growth Related Gene FGA1 in Rice

Author: WuZhaoXiang
Tutor: WangFeng
School: Fujian Agriculture and Forestry University
Course: Botany
Keywords: rice female sterile embryo sac T-DNA FGA1
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


We isolated a reproductive growth related gene, FGA1(female gametogenesis abortion 1), from a T-DNA insertional population of indica rice. The transgenic plants carrying a T-DNA insertion in the gene FGA1 displayed a phenomena that hygromycin resistance and sensitivity segregation ratios showed 1:1 byχ2-test. And the fga1/fga1 homozygous progeny was not found in 6 successive generations. Pollen fertility of fga1 mutant is normal, but the decline of ripening rate and the genotype of FGA1/fga1 exhibited the co-separetion according to the statistical analysis of ripening rate. Reciprocal crosses between FGA1/fga1 heterozygous plants and the wild types showed that the fga1 allele could not be transmitted through the female. All of these indicate that the mutant fga1 is a gametophyte ovary sterile mutant caused by T-DNA insertion.Cytologic observation showed us that pollen fertility of fga1 was as normal as the wild type minghui 86, but the development of female gamete was blocked. In the female gamete of fga1 mutant, we found , egg cell(E), synergids (S), antipodal cells and polar nuclei(P) appeared disaggregation one by one during the 8 nucleuses period, and the fertilization halted. Southern-blot and analysis of the DNA sequence flanking T-DNA showed that fga1 mutant carried one T-DNA insertion at code area of FGA1, which was located on chromosome 5. Above results fully explain that FGA1 are insertional mutants, and the infertility phenotype is not caused by the structure of T-DNA.The consequence of semi-quantitative reverse transcription-PCR indicated that FGA1 expressed during reproductive growth, so FGA1P::GUS was constructed to verify this result combined with GUS. FGA1 gene was isolated to construct expression vectors combined with pCXUN. Transgenic rice plants of the constructs were transform to rice mediated by agrobacterium. These results show that FGA1 is necessary for female gamete maturation. It may form a functional complex and regulate some gene or approach, which plays essential roles during the late stage of ovary formation.

Related Dissertations

  1. Study on Rice-Frog and Melon-Frog-fish Ecological Cultivation and Breeding Mode and the Nutrient Balance,S-0
  2. SCXC corporate culture construction,F270
  3. Monitoring of Insecticide Resistance and Resistance Risk Assessment for JS118 in the Rice Stem Borer, Chilo Suppressalis (Walker),S435.112.1
  4. Arthropod Diversity in Non-Crop Habitats at Margins of Paddy Fields of Different Types,S435.112
  5. Detection of the Resistance in Xanthomonas Oryzae PV. Oryzae and X. Oryzae PV. Oryzicola to Two Bactericides and the Assentment of Resistance Risk in Vitro,S435.111.4
  6. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  7. Study on Application of Red Yeast Rice in Fermented Sausage,TS251.65
  8. Studies on the Properties of Starch from Germinated Polished-rice-with-germ,TS235.1
  9. Research on Feed Safety of Transgenic Rice in Broiler,S831.5
  10. Treatment Efficiency of Entecavir and TACE in Patients with Primary Heptatic Carcinoma of the HBV Copying,R735.7
  11. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  12. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  13. Concentrations of Lipids in Grains of Japonica Rice: In Response to Nitrogen Fertilizer and in Relation to Physicochemical Properties of Rice Flour,S511.22
  14. Genetic Segregation Analysis and QTL Mapping as Well as Association Analysis for Panicle Angle Trait in Japonica Rice (Oryza Sativa L.),S511.22
  15. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  16. Bioinformatics Study of Motifs Within 3’UTR Region of Stress Response Genes and Functional Related Mirnas in Rice,Q943.2
  17. Effects of Seeding Stage on Source-Sink Relation in a Ratoon Rice Cropping System with Labor Reduced Cultivation in Northearstern Jiangxi Province,S511
  18. Effects of Different Water Management Methods on Cadimium Uptake and Translocation by Rice with Different Grain Accumulation Characteristics,S511
  19. Greenhouse Gases Emissions from Different Rotation Systems in Paddy Fields,S511
  20. Correlations between Physicochemical Properties, Molecular Structure of Rice Starch and Rice Eating Quality,S511
  21. Resistance Mechanism of Major Commercial Rice Varieties to RSV and Construction of Trangenic Rice to Fight Against Virus,S511

CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
© 2012 www.DissertationTopic.Net  Mobile