|
Objective To observe the advanced glycation end product receptor (RAGE) in varying degrees of acute lung injury (ALI) in rat plasma and bronchoalveolar lavage fluid (BALF) changes investigate RAGE value in the judgment of the severity of the ALI. Method 1. Establish different levels of lung injury model: ALI rat model of oleic acid - lipopolysaccharide second hit way replication. 30 SD rats were randomly divided into three groups: injuries caused by low-dose group, wounding the high-dose group, the control group, in which the low-dose group: oleic acid 0.1ml/kg, LPS 4mg/kg; injury high dose group: oil acid 0.15ml/kg, LPS 6mg/kg, control group with saline in place of the injured agent. Measurement the rat PaO 2 , lung tissue wet to dry weight ratio (W / D) in BALF of protein content, plasma and BALF tumor necrosis factor-α (TNF-α) levels, combined with the pathological changes evaluation of group of lung injury severity. Western blot assay of rat plasma and BALF RAGE content analysis of the relationship between and the degree of lung injury; detected in plasma, BALF vascular endothelial growth factor (VEGF) and angiopoietin -2 (Ang- 2) concentration, spearman rank correlation analysis of the above indicators of the degree of lung injury group correlation coefficient r value. 1 Two injured group showed PaO 2 decreased lung tissue W / D ratio and BALF protein content increased, the concentration of plasma and BALF TNF-α, compared with the control group are P lt; 0.05, differences were statistically significant, the high dose group of the more obvious changes, with the low-dose group p lt; 0.05, a statistically significant difference; pathological visible dose group of the low level of the high dose group of lung injury. Two injured plasma and BALF RAGE level rise, compared with the control group, P-lt; 0.05, the high-dose group BALF RAGE level in the low-dose group P-lt; 0.05, statistically significant; plasma RAGE the level difference between the low-dose group and high dose group was not statistically significant. Injury low-dose group, the plasma VEGF, Ang-2 concentration higher than the control group, the high-dose group, plasma VEGF, Ang-2 concentration increased further, both P lt; 0.05; the low doses injury in BALF VEGF concentration compared with the control group decreased VEGF concentration in the high-dose group BALF further decline have P lt; 0.05; in BALF of Ang-2 concentrations were no significant differences among groups. RAGE in the BALF the RAGE level and the plasma VEGF, Ang-2 levels and lung injury degree was positively correlated r order 0.943,0.667,0.863,0.877. Conclusion 1. Give the the gradient dose oleic - the lipopolysaccharide secondary combat the ALI rat model can be replicated to varying degrees. In oleic acid - lipopolysaccharide two-hit ALI rat model, with the severity of lung injury, the upward trend in BALF in RAGE level; RAGE levels and plasma in BALF RAGE, VEGF, Ang-2 and the degree of lung injury were positively correlated with the degree of lung injury, which the highest correlation coefficient in BALF RAGE level the degree of lung damage, better reaction ALI severity.
|