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Background: In recent years, more and more researchers are concerned about genetic research on human glioma become the current hot spot, especially for the homeobox gene in human glioma. Homeobox gene family containing a 183 nucleotide sequence of the common regulating genes, according to the the homeobox different, can be divided into different types, wherein the homeobox gene in mammals may clusters are arranged on the chromosome a class of gene expression at the front and rear axles, called Class I homeobox gene, also known as HOX genes (homeobox genes, homeobox genes). HOX family gene mutations cause abnormal ontogeny, as well as the formation of tissues and organs, and can even induce cells into malignant, and finally the formation of tumors. HOX genes can be divided into four clusters HOXA, B, C and D, which are located on four different chromosomes, they are closely related to the development of a variety of malignant tumors occur. In recent years, studies have shown that glioma cells and glioma tissues, HOXD10mRNA in as the glioma level increases expression was significantly decreased its abnormal expression that may have close ties with the occurrence and development of glioma. Objective: HOX family genes in human glioma cells and tissue expression mechanism of biological effect and gradually the number of research institutes reported, but HOXD10 expression in human glioma tissue and The clinical significance of the reported rare. The purpose of this experiment is to through HOXD10 gene expression in each grade glioma tissues and normal brain tissue, to infer a the HOXD10 gene glioma and development role, its expression level may become tumor detection indicators, in order to determine the tumor development and prognosis, to implement effective targeted therapy and individual therapy. Research methods: 1, using RT-PCR and Western blot analysis of the expression of the normal brain tissue and glioma tissues HOXD. 2, RT-PCR detection of tissue HOXD10 mRNA expression level: each grade gliomas and normal brain tissue extract the total RNA, RNA purity measured, total RNA RT, the resultant cDNA row reverse transcription PCR amplification, analysis and detection HOXD10 mRNA expression level. Western blot detection of tissue HOXD10 protein level expression: all levels glioma tissues and normal brain tissue lysis, gel electrophoresis protein isolate, detect tissue HOXD10 protein level of expression. Results: 1, normal tissue HOXD10 mRNA was highly expressed in amount Ⅲ ~ Ⅳ grade gliomas HOXD10 mRNA expression was significantly lower than Ⅰ ~ Ⅱ grade glioma. HOXD10 protein expression in normal tissues was significantly higher than glioma, and the HOXD10 protein expression in glioma tissue with glioma level increased, the expression level showed a decreasing trend. Research conclusions: HOXD10 gene was highly expressed in normal brain tissue showed low expression in human glioma tissues and HOXD10 gene expression in glioma tissue with glioma increased levels of a downward trend, and was negatively correlated with the degree of malignancy of glioma. This prompted HOXD10 may be closely related to the with glioma incidence of development, HOXD10 may be a tumor suppressor gene, and as a glioma development and prognosis indexes.
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